Evidence map›Paper›PMID 40673314›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2025

[Pirfenidone inhibits bladder cancer xenograft growth in mice by regulating regulatory T cells].

Hongbo Zhang, Mengyu Yan, Jiandong Zhang, Peiwang Sun, Rui Wang, Yuanyuan Guo

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hongbo ZhangDepartment of Urology, First Affiliated Hospital of Bengbu Medical University, Bengbu 233040, China.
Mengyu YanDepartment of Urology, First Affiliated Hospital of Bengbu Medical University, Bengbu 233040, China.
Jiandong ZhangDepartment of Urology, First Affiliated Hospital of Bengbu Medical University, Bengbu 233040, China.
Peiwang SunDepartment of Urology, First Affiliated Hospital of Bengbu Medical University, Bengbu 233040, China.
Rui WangDepartment of Urology, First Affiliated Hospital of Bengbu Medical University, Bengbu 233040, China.
Yuanyuan GuoDepartment of Urology, First Affiliated Hospital of Bengbu Medical University, Bengbu 233040, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo investigate the inhibitory effect of pirfenidone (PFD) on growth of bladder cancer xenograft and its regulatory effect on Treg cells in tumor-bearing mice.

methodsThirty-two C57BL/6 mice bearing ectopic bladder tumors were randomized into control and PFD groups (

resultsCompared with the control group, the PFD-treated mice exhibited significantly lower tumor growth rate with smaller tumor volumes at day 21, along with improved survival at day 28. Immunohistochemistry revealed no significant differences in the infiltration of CD3⁺ and CD8⁺ cells between the two groups, but the percentages of CD4⁺ and FOXP3⁺ cells were significantly lower in the tumors of PFD-treated mice. Flow cytometric analysis confirmed a decrease in CD4⁺CD25⁺FOXP3⁺ Treg cells in the tumors from PFD-treated mice, which also had reduced expression levels of IL-2, IL-10 and IL-35 mRNAs in the tumors. No significant differences were found in Treg cell populations or cytokine expressions in the spleen tissues between the two groups. HE staining showed obvious organ damage in neither of the groups.

conclusionsPFD inhibits bladder cancer growth and enhances survival of tumor-bearing mice possibly by suppressing Treg cells in the tumor microenvironment.

Indexed as

T-Lymphocytes, RegulatoryUrinary Bladder NeoplasmsAnimalsCell Line, TumorFemaleInterleukin-10Interleukin-2InterleukinsMiceMice, Inbred C57BLXenograft Model Antitumor AssaysIL10 protein, mouseInterleukin-10Interleukin-2interleukin-35, mouseInterleukinsbladder cancerimmune microenvironmentpirfenidoneregulatory T cells

Identifiers

PMID40673314
PMCPMC12268924

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.