Evidence map›Paper›PMID 40671676›Full record

ArticleMolecular therapy : the journal of the American Society of Gene Therapy2025

Disruption of the Nucleolin-CXCR4 interaction by the DNA aptamer HY-4 halts colorectal cancer metastasis.

Yunyi Liu, Yatao Wu, Changyue Yuan, Bei Hu, Yuxi Xu, Hailong Ou, Juan Li, Dan Qi, Bi Shi, Yiliang Wu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yunyi LiuState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China; Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China.
Yatao WuState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Changyue YuanState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Bei HuState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Yuxi XuState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Hailong OuState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Juan LiState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Dan QiDepartment of Neurosurgery and Neuroscience Institute, Baylor Scott & White Health, Temple, TX 76508, USA; Department of Neurosurgery, Baylor College of Medicine, Temple, TX 76508, USA.
Bi ShiState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Yiliang WuState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Jason H HuangDepartment of Neurosurgery and Neuroscience Institute, Baylor Scott & White Health, Temple, TX 76508, USA; Department of Neurosurgery, Baylor College of Medicine, Temple, TX 76508, USA.
Erxi WuDepartment of Neurosurgery and Neuroscience Institute, Baylor Scott & White Health, Temple, TX 76508, USA; Department of Neurosurgery, Baylor College of Medicine, Temple, TX 76508, USA; College of Medicine and Irma Lerma Rangel College of Pharmacy, Texas A & M University, College Station, TX 77843, USA; Livestrong Cancer Institutes and Department of Internal Medicine, Dell Medical School, the University of Texas at Austin, Austin, TX 78712, USA. Electronic address: erxi.wu@bcm.edu.
Xiaoxiao HuState Key Laboratory of Chemo/Biosensing and Chemometrics, Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province, Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, Hunan 410082, China; Shenzhen Research Institute, Hunan University, Shenzhen, Guangdong 518000, China; Research Institute of Hunan University in Chongqing, Chongqing 401120, China; Innovation Institute of Industrial Design and Machine Intelligence, Quanzhou-Hunan University, Quanzhou 362006, China. Electronic address: xxhu@hnu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colorectal cancer, characterized by its aggressive metastatic behavior and often poor prognosis, urgently necessitates improved diagnostic and therapeutic strategies. This study introduces HY-4, an aptamer developed via a non-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method, which shows exceptional specificity and affinity for nucleolin (NCL), a highly expressed oncogenic protein in various cancers. With remarkable biocompatibility, HY-4 significantly reduces the migration and invasion of LoVo cancer cells by disrupting the NCL-CXCR4 interaction, a pivotal pathway in cancer metastasis. This disruption highlights the potential of HY-4 to inhibit cancer metastasis without producing adverse effects. Notably, HY-4 distinguishes itself from conventional NCL-targeting aptamers by avoiding the typical G-quadruplex structure and instead adopting a unique non-G-quadruplex conformation to create a novel binding site. This unique structural conformation provides critical insight into NCL-targeting mechanisms and could profoundly influence the landscape of targeted cancer therapy. By utilizing advanced techniques such as circular dichroism spectroscopy and molecular docking simulations, we obtained detailed insights into the structural dynamics and inhibitory interactions of HY-4, thereby deepening our understanding of the crucial structure-activity relationships in NCL-targeted drug development. In summary, HY-4 represents a promising advancement in targeted therapy, potentially heralding a paradigm shift in the treatment and diagnosis of colorectal cancer.

Indexed as

Aptamers, NucleotideColorectal NeoplasmsPhosphoproteinsReceptors, CXCR4RNA-Binding ProteinsBinding SitesCell Line, TumorCell MovementG-QuadruplexesHumansMolecular Docking SimulationNeoplasm MetastasisNucleolinProtein BindingSELEX Aptamer TechniqueAptamers, NucleotideCXCR4 protein, humanNucleolinPhosphoproteinsReceptors, CXCR4RNA-Binding Proteinsaptamercolorectal cancerCXCR4metastasisNCL

Identifiers

PMID40671676
PMCPMC12848210

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.