Evidence map›Paper›PMID 40671648›Full record

ArticleMolecular ecology resources2025

Multi-Tool Marine Metabarcoding Bioassessment for Baselining and Monitoring Species and Communities in Kelp Habitats.

Giulia Maiello, Marilla R Lippert, Erika F Neave, Erik A Hanson, Stephen R Palumbi, Stefano Mariani

Abstract read
In one paragraph

Article in Molecular ecology resources, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Giulia MaielloSchool of Biological and Environmental Sciences, Liverpool John Moores University, Liverpool, UK.ORCID https://orcid.org/0000-0002-0431-4205
Marilla R LippertHopkins Marine Station, Department of Biology, Stanford University, Pacific Grove, California, USA.
Erika F NeaveSchool of Biological and Environmental Sciences, Liverpool John Moores University, Liverpool, UK.ORCID https://orcid.org/0000-0002-0410-0338
Erik A HansonHopkins Marine Station, Department of Biology, Stanford University, Pacific Grove, California, USA.
Stephen R PalumbiHopkins Marine Station, Department of Biology, Stanford University, Pacific Grove, California, USA.
Stefano MarianiSchool of Biological and Environmental Sciences, Liverpool John Moores University, Liverpool, UK.ORCID https://orcid.org/0000-0002-5329-0553

Funding

Oceankind LabsThe Gordon and Betty Moore FoundationThe Pew Charitable Trusts under the Lenfest Ocean Program
6 · The paper itself

Abstract

The astonishing biological diversity found in Californian kelp forests requires efficient and robust monitoring tools to better understand ecological trends and mitigate against loss or disruption of ecosystem services due to human pressure and climate changes. With environmental DNA (eDNA) metabarcoding becoming a popular biodiversity assessment approach, we set out to evaluate a combination of powerful, rapid and sustainable eDNA solutions for characterising marine community composition in kelp-dominated habitats along the central California coast, in the newly proposed Chumash Heritage National Marine Sanctuary. We employed and compared the efficiency of several eDNA collection approaches, including 'traditional' surface water filtration, the collection of organisms encrusting cobble rocks and various deployments of an artificial passive sampler, the metaprobe (i.e., attached to divers, dangled from a boat and cast from the shore using a fishing rod). By combining the information from fish specific (Tele02 12S) and universal metazoan (COI) markers, we 'captured' 501 unique marine taxa, belonging to at least 36 phyla, over 400 of which were identified to genus/species level, and including 52 vertebrate species typical of Californian kelp forest ecosystems. Despite differences in the type of biodiversity returned by the tested sampling methods, the overall community structure of the surveyed area was highly spatially structured and strongly influenced by the biogeographic break around Point Conception (Humqaq). We discuss the benefits of integrating eDNA metabarcoding in existing monitoring programs and devising a reproducible approach to monitor faunal changes in kelp forest habitats and beyond.

Indexed as

Aquatic OrganismsBiodiversityBiotaDNA Barcoding, TaxonomicDNA, EnvironmentalKelpMetagenomicsAnimalsCaliforniaEcosystemDNA, Environmentalbiomonitoringenvironmental DNAkelp forestsmarine communitiesmarine protected areas

Identifiers

PMID40671648
PMCPMC12415811

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.