Evidence map›Paper›PMID 40671144›Full record

ArticleJournal of animal science and biotechnology2025

SIRT3 mediates CPT2 delactylation to enhance mitochondrial function and proliferation in goat granulosa cells.

Shuaifei Song, Mingzhi Yang, Jiayue Li, Yaru Li, Lei Wang, Shiyi Yao, Zihan Wang, Qiuyan Li, Yanguo Han, Dejun Xu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Oxidative Stress andInternational journal of molecular sciences · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shuaifei SongChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Mingzhi YangChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Jiayue LiChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Yaru LiChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Lei WangChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Shiyi YaoChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Zihan WangChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Qiuyan LiChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Yanguo HanChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Dejun XuChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
Zhongquan ZhaoChongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing, 400715, China. zhaozhongquan@swu.edu.cn.ORCID http://orcid.org/0000-0002-6268-8539

Funding

National Key Research and Development Program of China 2022YFD1300202Technology Innovation and Application Development Special Project of Chongqing cstc2021jscx-gksbX0008
6 · The paper itself

Abstract

backgroundReproductive efficiency in goats is closely linked to the healthy development of follicles, with the proliferation of ovarian granulosa cells (GCs) playing a crucial role in this process. Sirtuin 3 (SIRT3), an enzyme that catalyzes post-translational modifications (PTMs) of proteins, is known to regulate a variety of mitochondrial metabolic pathways, thereby affecting cell fate. However, the specific effect of SIRT3 on the follicular development process remains unclear. Therefore, this study aimed to investigate the regulatory role of SIRT3 in the mitochondrial function and proliferation of goat GCs, as well as the underlying mechanisms involved.

resultsIn this study, GCs from small follicles in goat ovaries presented increased proliferative potential and elevated SIRT3 expression levels compared with those from large follicles. In vitro, SIRT3 overexpression enhanced mitochondrial function, promoted proliferation and inhibited apoptosis in GCs. Correspondingly, the inhibition of SIRT3 led to the opposite effects. Notably, SIRT3 interacted with carnitine palmitoyl transferase 2 (CPT2) and stabilized the CPT2 protein by mediating delactylation, which prolonged the half-life of CPT2 and prevented its degradation. Further investigation revealed that CPT2 overexpression enhanced fatty acid β-oxidation and mitochondrial function in GCs. Additionally, CPT2 promoted the proliferation of GCs by increasing the protein levels of β-catenin and its downstream target, cyclin D1 (CCND1). However, this effect was reversed by 3-TYP (a SIRT3 inhibitor).

conclusionsSIRT3 stabilizes CPT2 protein expression through delactylation, thereby enhancing mitochondrial function and the proliferative capacity of GCs in goats. This study provides novel insights into the molecular mechanisms and regulatory pathways involved in mammalian follicular development.

Indexed as

CPT2DelactylationMitochondrial functionOvarian granulosa cellsProliferationSIRT3

Identifiers

PMID40671144
PMCPMC12269156

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.