ArticleEuropean journal of medical research2025
High-fat diet activates pyroptosis of retinal pigment epithelial cells in aged TgAPPswePS1 transgenic mice.
Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Impact of a High-Fat Diet on Eye Health.Nutrients · 2025Pooled it
- Correction: High-fat diet activates pyroptosis of retinal pigment epithelial cells in aged TgAPPswePS1 transgenic mice.European journal of medical research · 2025Article
- Amyloid-β-induced pyroptosis drives retinal neurodegeneration in a TgAPPswePS1 mouse model.International journal of ophthalmology · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
purposeThis study assesses the impact of high-fat diet on retinal pigment epithelium (RPE) of aged TgAPPswePS1 transgenic mice, focusing on the involvement of RPE cell pyroptosis.
methodsTwenty-four TgAPPswePS1 transgenic mice (18 months) was randomly divided into Tg group (n = 12) and Fat group (n = 12). Mice in Fat group were fed with high-fat diet consisting of 81.85% standard chow, supplemented with 1% cholesterol, 015% cholic acid, and 17% hydrogenated vegetable oil. Another 12 wild-type C57BL/6J mice were serve as control group. The fundus was examined through Micron IV. The eyes of mice were removed for paraffin-embedding and sectioning. HE staining was carried out to observe the structure of retina and measure retinal thickness. The expressions of amyloid-beta (Aβ), NOD-like receptor thermal protein domain associated protein 3 (NLRP3), Caspase-1, gasdermin D (GSDMD), IL-1β and IL-18 in RPE, as well as the number of RPE were detected.
resultsThe RPE in Fat group showed obvious Aβ accumulation (p < 0.0001). Compared with the Tg group, the thickness of retina in the Fat group was significantly reduced (t = 5, p = 0.0075), and the number of RPE was statistically decreased (t = 4.243, p = 0.0132). In addition, the expressions of pyroptosis-related proteins NLRP3, Caspase-1, GSDMD, IL-1β and IL-18 in RPE were significantly increased in Fat group (p < 0.05).
conclusionsHigh-fat diet leads to Aβ accumulation in the RPE of aged TgAPPswePS1 transgenic mice, causes RPE damage, in which RPE cell pyroptosis may play a crucial role.
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Registered trials
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