Evidence map›Paper›PMID 40670977›Full record

ArticleBMC veterinary research2025

The combined effects of extended feeding with a high level of Arthrospira platensis and a commercial enzyme mix or porcine pancreatin on broilers' blood cells, plasma metabolites and liver lipid profile.

Paula A Lopes, Cristina M Alfaia, Maria P Spínola, Rui M A Pinto, José M Pestana, Mónica M Costa, J C Tavares, Miguel P Mourato, Beatriz Tavares, Daniela F P Carvalho and 3 more

Abstract read
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Paula A Lopes *Faculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisboa, 1300-477, Portugal.
Cristina M Alfaia *Faculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisboa, 1300-477, Portugal.
Maria P SpínolaFaculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisboa, 1300-477, Portugal.
Rui M A PintoLaboratório de Análises Clínicas Dr. Joaquim Chaves, JCS, Avenida General Norton de Matos, Miraflores, Algés, 1495-148, Portugal.
José M PestanaFaculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisboa, 1300-477, Portugal.
Mónica M CostaFaculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisboa, 1300-477, Portugal.
J C TavaresInstituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisboa, 1349-017, Portugal.
Miguel P MouratoEnvironment, Agriculture and Food, Instituto Superior de Agronomia, Associated Laboratory TERRA, LEAF - Linking Landscape, Universidade de Lisboa, Tapada da Ajuda, Lisboa, 1349-017, Portugal.
Beatriz TavaresInstituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisboa, 1349-017, Portugal.
Daniela F P CarvalhoEnvironment, Agriculture and Food, Instituto Superior de Agronomia, Associated Laboratory TERRA, LEAF - Linking Landscape, Universidade de Lisboa, Tapada da Ajuda, Lisboa, 1349-017, Portugal.
Cátia F MartinsFaculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisboa, 1300-477, Portugal.
Madalena LordeloEnvironment, Agriculture and Food, Instituto Superior de Agronomia, Associated Laboratory TERRA, LEAF - Linking Landscape, Universidade de Lisboa, Tapada da Ajuda, Lisboa, 1349-017, Portugal.
José A M PratesFaculdade de Medicina Veterinária, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Lisboa, 1300-477, Portugal. japrates@fmv.ulisboa.pt.

Funding

Fundação para a Ciência e a Tecnologia UIDB/00276/2020Portugal2020 P2020/17/SI/70114/2019
6 · The paper itself

Abstract

backgroundThe increasing global demand for sustainable and eco-friendly protein alternatives in poultry production has led to the exploitation of unconventional feed ingredients, such as microalgae. This study is novel for its exploration of the extended feeding effects (from day 7 to day 35) of a high inclusion level (15%) of Arthrospira platensis (Spirulina) in broiler chickens' diet, either alone or supplemented with enzymes, primarily peptidases (EC 3.4). The study assessed the impact on the blood cells, metabolic status and the hepatic content of lipids, pigments and minerals. One hundred and twenty Ross 308 male chickens were distributed into 40 battery brooders, housing three birds per cage. Initially, the chickens were given ad libitum access to a standard corn and soybean meal-based diet for the first seven days. Subsequently, from day 7 to 35, they were assigned to one of four experimental diets: a control diet based on corn and soybean (control group, n = 10), a diet incorporating 15% A. platensis (SP group, n = 10), a diet incorporating 15% A. platensis supplemented with 0.025% of the commercial enzyme mix VemoZyme

resultsThe 15% inclusion of A. platensis negatively impacted the birds' growth performance by decreasing the final body weight (p < 0.001), body weight gain (p < 0.001) and average daily feed intake (p < 0.001), while increasing the feed conversion ratio (p = 0.001). This high level of A. platensis incorporation did not change the haematological profile but raised blood lipid levels. However, these increases were successfully normalized by supplementing the diet with the enzyme mix VemoZyme

conclusionsOverall, data suggest that the impact of A. platensis on blood and liver measurements outweighs the effect of enzyme supplements, VemoZyme

Indexed as

ChickensSpirulinaAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsBlood CellsDietDietary SupplementsLipid MetabolismLipidsLiverMaleSwineLipidsArthrospira platensisBroilersHepatic lipidsLong-term feedingPeptidasesPlasma metabolites

Identifiers

PMID40670977
PMCPMC12269105

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.