Evidence map›Paper›PMID 40670899›Full record

ReviewMolecular biotechnology2026

Advances in Tissue Culture-Free Genetic Engineering and Genome Editing of Peanut.

Tariq Alam

Abstract readReview
In one paragraph

Review in Molecular biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Tariq AlamDepartment of Plant and Environmental Sciences, Clemson University Pee Dee Research and Education Center, Florence, SC, 29506, USA. talam@clemson.edu.ORCID http://orcid.org/0000-0002-7147-1542

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant transformation and genome editing are pivotal in advancing peanut biotechnology, yet traditional tissue culture-dependent methods are hindered by lengthy protocols, genotype dependency, and somaclonal variation. CRISPR/Cas technologies have revolutionized breeding by enabling precise, multiplex genome editing to improve traits such as disease resistance, allergen reduction, seed quality, and yield. However, variable transformation efficiencies and chimerism remain challenges. This review examines emerging tissue culture-independent techniques such as nanoparticle-based delivery, viral vectors, pollen magnetofection, pollen tube injection, node injection, and vacuum infiltration that offer rapid, cost-effective gene transfer. It also highlights the integration of high-throughput screening, robust selection markers, and automation, including robotics and advanced imaging, to refine transformation pipelines. These methodological breakthroughs promise to overcome current limitations and accelerate the development of improved peanut cultivars for sustainable agriculture.

Indexed as

ArachisGene EditingGenetic EngineeringCRISPR-Cas SystemsGenome, PlantPlants, Genetically ModifiedTissue Culture TechniquesCRISPR/Cas9 genome editingHigh-throughput transformationMagnetofectionPollenTissue culture-free methods

Identifiers

PMID40670899
PMCPMC13053326

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.