Evidence map›Paper›PMID 40670710›Full record

ArticleNature biotechnology2026

Comprehensive taxonomic identification of microbial species in metagenomic data using SingleM and Sandpiper.

Ben J Woodcroft, Samuel T N Aroney, Rossen Zhao, Mitchell Cunningham, Joshua A M Mitchell, Rizky Nurdiansyah, Linda Blackall, Gene W Tyson

Abstract read
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In one paragraph

Article in Nature biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed.

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  9. International journal of systematic and evolutionary microbiology · 2026
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  14. Ecological plasticity ofMicrobiology spectrum · 2026
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  20. Physiology, functional genomics, and proteomics ofApplied and environmental microbiology · 2026
    Article

12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ben J WoodcroftCentre for Microbiome Research, School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute, Woolloongabba, Queensland, Australia. b.woodcroft@qut.edu.au.ORCID http://orcid.org/0000-0003-0670-7480
Samuel T N AroneyCentre for Microbiome Research, School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute, Woolloongabba, Queensland, Australia.ORCID http://orcid.org/0000-0001-9806-5846
Rossen ZhaoCentre for Microbiome Research, School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute, Woolloongabba, Queensland, Australia.ORCID http://orcid.org/0000-0002-7003-2791
Mitchell CunninghamSchool of BioSciences, The University of Melbourne, Melbourne, Victoria, Australia.
Joshua A M MitchellCentre for Microbiome Research, School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute, Woolloongabba, Queensland, Australia.
Rizky NurdiansyahCentre for Microbiome Research, School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute, Woolloongabba, Queensland, Australia.
Linda BlackallSchool of BioSciences, The University of Melbourne, Melbourne, Victoria, Australia.ORCID http://orcid.org/0000-0002-8848-7698
Gene W TysonCentre for Microbiome Research, School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute, Woolloongabba, Queensland, Australia.ORCID http://orcid.org/0000-0001-8559-9427

Funding

Amazon Web Services (AWS) n/aDepartment of Education and Training | Australian Research Council (ARC) FT210100521Google n/aNational Science Foundation (NSF) 2022070U.S. Department of Energy (DOE) DE-SC0004632U.S. Department of Energy (DOE) DE-SC0010580U.S. Department of Energy (DOE) DE-SC0016440
6 · The paper itself

Abstract

Determining the taxonomy and relative abundance of microorganisms in metagenomic data remains technically challenging. Here we present 'SingleM', which estimates community composition using conserved regions within universal marker genes. By accurately incorporating species lacking genomic representation, we show that unknown species dominate in most environmental microbial communities. Our website 'Sandpiper' collates microbial community profiles from 248,559 publicly available metagenomes.

Indexed as

BacteriaMetagenomeMetagenomicsDatabases, GeneticPhylogeny

Identifiers

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.