ArticleProceedings of the National Academy of Sciences of the United States of America2025
ADPact: A versatile sensor for ADP-F-actin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- ADPact: A versatile sensor for ADP-F-actin.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The dynamic organization of the actin cytoskeleton, crucial for numerous cellular processes, is intricately regulated by the nucleotide state of actin filaments (F-actin). Visualization tools for specifically detecting ADP-bound F-actin, however, remain limited. Here, we introduce ADPact, a 20-amino-acid peptide that selectively binds to ADP-F-actin. ADPact allows for the precise visualization of ADP-F-actin structures both in vitro and in eukaryotic cells, particularly under energy stress conditions where ADP-/ATP-actin ratio increases. Importantly, ADPact and its fluorescent fusion variant, ADPact-GFP, do not disrupt actin dynamics, ensuring accurate monitoring of cytoskeletal reorganization. Our findings provide a powerful tool for studying actin dynamics and offer fresh insights into the regulation of the cytoskeleton under physiological and pathological conditions.
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Registered trials
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