ArticleJournal of assisted reproduction and genetics2025
Evaluating non-invasive Preimplantation Genetic Testing for Aneuploidy (niPGT-A) from spent culture medium: prolonged embryo culture to day 6 increases informativity without negatively impacting quality and live birth rates.
Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeEmbryos release cell-free DNA into spent culture medium (SCM), mostly from day 5 to day 6, making extended culture to day 6 (something done in IVF only for slow embryos) a preferred option in non-invasive preimplantation genetic testing for aneuploidy (niPGT-A). The impact of day 6 culture on embryo quality remains unclear. This study investigates whether prolonged culture to day 6 in niPGT-A improves informativity without compromising embryo quality and live birth rates (LBR).
methodsIn this retrospective cohort study, we investigate the impact of day 6 culture on embryo quality and LBR. After preliminary concordance assessment on 18 embryos, a total of 336 SCM from day six blastocysts were collected. According to the prioritisation criteria applied, 103 embryos were transferred in 91 frozen embryo transfer cycles (January 2021-February 2024). Informativity rates in day 5/6, levels of expansion/quality, female age impact and effects of prolonged culture to day 6 on embryo quality were assessed.
resultsHigher informativity rate was observed in day 6 versus day 5 blastocysts (p < 0.001). Informativity was unaffected by different age groups (p = 0.22), blastocyst expansion (p = 0.51), blastocyst quality (p = 0.67), or incubator setting (p = 0.74). Day 6 prolonged culture did not compromise embryo viability and quality and better quality embryos had higher probability of euploidy (p = 0.01). We report a LBR of 33.3%, higher than the reported 10% by HFEA for a similar age group (40-42 years).
conclusionsDay 6 extended culture in niPGT-A improves informativity and does not compromise embryo viability and quality. LBR significantly increased compared to international benchmarks of non-tested embryos.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.