Evidence map›Paper›PMID 40668463›Full record

ArticleMolecular biology reports2025

Toxoplasma gondii soluble antigen (STAg) induces resistance against Mycobacterium tuberculosis in a human monocyte cell line via P2X7 receptor.

Parisa Ilbeigi, Javid Sadraei, Fatemeh Ghaffarifar, Seyed Davar Siadat, Hamed Mirjalali

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Parisa IlbeigiDepartment of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Javid SadraeiDepartment of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. sadraeij@modares.ac.ir.
Fatemeh GhaffarifarDepartment of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Seyed Davar SiadatMicrobiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
Hamed MirjalaliFoodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. hamed_mirjalali@hotmail.com.ORCID http://orcid.org/0000-0002-2568-9022

Funding

Shahid Beheshti University of Medical Sciences RIGLD- 1059
6 · The paper itself

Abstract

backgroundThe control of T. gondii in macrophages is mediated by the P2X7 receptor. The P2X7 receptor plays a crucial role in defense against Mycobacterium tuberculosis (M.tb), as well. Current study aimed to investigate the immunity induced by T. gondii soluble total antigen (STAg) against M.tb via P2X7R in human monocyte cell line.

methodsThe STAg was prepared from 1.6 × 10

resultsThe qReal-time PCR analysis showed overexpression of the ATG5 gene in P2X7R siRNA, scramble siRNA, and STAg/siRNA and ATG7 in THP-1 cell line treated by STAg groups, while they were suppressed in STAg/siRNA/ M.tb and STAg/ M.tb groups. The expression of ATG16 was significantly overexpressed in STAg group, while it was suppressed in treated wells by STAg/siRNA, STAg/siRNA/M.tb, STAg/M.tb, and M.tb. The number of entered M.tb in STAg/M.tb was significantly lower than M.tb group. The number of M.tb in P2X7R-knockdown THP-1 cells was significantly higher than scramble siRNA and STAg. In addition, the number of the bacteria in STAg/siRNA was significantly lower than P2X7R siRNA group.

conclusionOur study showed that sensing macrophages by T. gondii STAg may induce levels of immunity against M.tb. In addition, our findings support available data, indicating potential role of P2X7R in macrophage against T. gondii and M.tb.

Indexed as

Antigens, ProtozoanMycobacterium tuberculosisReceptors, Purinergic P2X7ToxoplasmaCell LineHumansMacrophagesMonocytesRNA, Small InterferingTHP-1 CellsTuberculosisAntigens, ProtozoanP2RX7 protein, humanReceptors, Purinergic P2X7RNA, Small InterferingMycobacterium tuberculosisP2X7 receptorTHP-1 cell lineToxoplasma gondii

Identifiers

PMID40668463

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