Evidence map›Paper›PMID 40668238›Full record

ArticleBiotechnology letters2025

A novel P450 enzyme assay utilizing an NADP

Sifan Shangguan, Taichang Wang, Di Zhao, Guobin Zhang, Yisang Zhang, Ruiming Wang, Junqing Wang, Jing Su

Abstract read
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In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sifan ShangguanState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, 250353, Shandong, Republic of China.
Taichang WangState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, 250353, Shandong, Republic of China.
Di ZhaoState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, 250353, Shandong, Republic of China.
Guobin ZhangState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, 250353, Shandong, Republic of China.
Yisang ZhangState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, 250353, Shandong, Republic of China.
Ruiming WangState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, 250353, Shandong, Republic of China.
Junqing WangState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, 250353, Shandong, Republic of China.ORCID http://orcid.org/0000-0001-9862-2366
Jing SuState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, 250353, Shandong, Republic of China. sj_12346@163.com.ORCID http://orcid.org/0000-0002-8604-6629

Funding

Key innovation Project of Qilu University of Technology 2024ZDZX03National Key Research and Development Program of China 2023YFD1300700Natural Science Foundation of Shangdong Province ZR2023MC089Research and Development Plan in Shangdong Province 2023CXGC010714
6 · The paper itself

Abstract

purposeHigh-throughput screening methods for cytochrome P450 enzymes (P450s), such as colorimetric, mass spectrometric, and fluorescence-based assays, often face limitations in throughput, real-time monitoring, and versatility.

methodsTo address these challenges, we developed a novel biosensor leveraging glucose-6-phosphate dehydrogenase and Bimolecular Fluorescence Complementation for real-time monitoring of intracellular NADP

resultsThe sensor exhibited a linear NADP

conclusionThe developed biosensor demonstrates its potential as a robust, real-time screening tool for P450 enzyme studies.

Indexed as

Biosensing TechniquesCytochrome P-450 Enzyme SystemEnzyme AssaysNADPEscherichia coliGlucosephosphate DehydrogenaseCytochrome P-450 Enzyme SystemGlucosephosphate DehydrogenaseNADPCRISPR/Cas9HemeNADP + sensorP450 enzyme detection

Identifiers

PMID40668238

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.