Evidence map›Paper›PMID 40667655›Full record

ArticleBiology open2025

Inducing bursicon expression using 20-hydroxyecdysone (20E) increased immune response in Macrobrachium rosenbergii against Aeromonas hydrophila.

Arnon Pudgerd, Sukanya Saedan, Thanapong Kruangkum, Kallaya Sritunyalucksana, Sirilak Sanpa, Sudthiporn Somnet, Rapeepun Vanichviriyakit, Charoonroj Chotwiwatthanakun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Arnon PudgerdDivision of Anatomy, School of Medical Science, University of Phayao, Muang, Phayao 56000, Thailand.ORCID 0000-0003-4198-7748
Sukanya SaedanCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
Thanapong KruangkumCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.ORCID 0000-0003-3191-5564
Kallaya SritunyalucksanaAquatic Animal Health Research Team (AQHT), Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Bangkok 10400, Thailand.
Sirilak SanpaUnit of Excellence in Plant and Aquaculture, University of Phayao, Muang, Phayao 56000, Thailand.
Sudthiporn SomnetDivision of Anatomy, School of Medical Science, University of Phayao, Muang, Phayao 56000, Thailand.
Rapeepun VanichviriyakitCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.ORCID 0000-0002-8712-7578
Charoonroj ChotwiwatthanakunCenter of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.ORCID 0000-0001-9137-8444

Funding

Fundamental Fund, the Thailand Science Research and Innovation Fund, and the University of Phayao. FF64-UoE24Fundamental Fund, the Thailand Science Research and Innovation Fund, and the University of Phayao. FF66-RIM062Mahidol UniversityNational Research Council of Thailand (NRCT) under the High-Potential Research Team Grant Program N42A650869
6 · The paper itself

Abstract

A bursicon (burs) is a neurohormone that regulates cuticle tanning after molting, as well as the immune response, in insects and crustaceans. This study investigated the relationship between ecdysteroids, burs expression and immune regulation following 20-hydroxyecdysone (20E) injection in Macrobrachium rosenbergii. Burs subunits (burs α and burs β) were highly expressed in the thoracic ganglia during the late pre-molt stage (D3). Significant upregulation of the burs α and burs β, anti-lipopolysaccharide factor (ALF) and crustacean hematopoietic factor (CHF) genes accompanied an increase in the hemocyte concentration. The efficacy of immune enhancement for disease protection from 20E injection showed that 20E treatment upregulated burs genes in thoracic and abdominal ganglia. ALF and CHF expression and phenol oxidase activity were significantly increased. The hemocyte proliferation percentage in hematopoietic tissue increased 12 h post-infection, while circulating hemocytes increased significantly within the first 24 h. Administering 20E decreased mortality in Aeromonas hydrophila-challenged prawns. This study demonstrated that the ecdysteroid 20E stimulated burs expression and improved the immune response to bacterial challenge, suggesting that this hormone plays a role in regulating the immune system during ecdysis.

Indexed as

Aeromonas hydrophilaEcdysteroneGene Expression RegulationGram-Negative Bacterial InfectionsInvertebrate HormonesPalaemonidaeAnimalsHemocytesImmunity, InnatebursiconEcdysteroneInvertebrate Hormones20-hydroxyecdysoneAeromonas hydrophilaBursicon neurohormoneImmune responseMacrobrachium rosenbergii

Identifiers

PMID40667655
PMCPMC12309897

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.