ArticleThe Journal of cell biology1985
Exocytosis of sea urchin egg cortical vesicles in vitro is retarded by hyperosmotic sucrose: kinetics of fusion monitored by quantitative light-scattering microscopy.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 89 citations in OpenAlex.
- Unraveling the mechanisms of calcium-dependent secretion.The Journal of general physiology · 2019Review
- Resin embedded multicycle imaging (REMI): a tool to evaluate protein domains.Scientific reports · 2016Article
- Quantification of exocytosis kinetics by DIC image analysis of cortical lawns.Journal of chemical biology · 2014Article
- A new approach to the molecular analysis of docking, priming, and regulated membrane fusion.Journal of chemical biology · 2011Article
- The Gaussian curvature elastic energy of intermediates in membrane fusion.Biophysical journal · 2008Article
- Osmotic regulation of Rab-mediated organelle docking.Current biology : CB · 2008Article
- A kinetic analysis of calcium-triggered exocytosis.The Journal of general physiology · 2001Article
- Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.The Journal of physiology · 1999Review
- Biochemical and functional studies of cortical vesicle fusion: the SNARE complex and Ca2+ sensitivity.The Journal of cell biology · 1998Article
- Submaximal responses in calcium-triggered exocytosis are explained by differences in the calcium sensitivity of individual secretory vesicles.The Journal of general physiology · 1998Article
- Poisson-distributed active fusion complexes underlie the control of the rate and extent of exocytosis by calcium.The Journal of cell biology · 1996Article
- Application of a membrane fusion assay for rapid drug screening.Pharmaceutical research · 1995Article
- Proteins on exocytic vesicles mediate calcium-triggered fusion.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Calcium requirements for secretion in bovine chromaffin cells.The Journal of physiology · 1992Article
- Effects of osmotic stress on mast cell vesicles of the beige mouse.The Journal of membrane biology · 1992Article
- Characterization of constitutive exocytosis in the yeast Saccharomyces cerevisiae.The Journal of membrane biology · 1991Article
- An electron-microscope and freeze-fracture study of the egg cortex of Brachydanio rerio.Cell and tissue research · 1991Article
- Is swelling of the secretory granule matrix the force that dilates the exocytotic fusion pore?Biophysical journal · 1991Article
- Reassociation of cortical secretory vesicles with sea urchin egg plasma membrane: assessment of binding specificity.The Journal of membrane biology · 1990Article
- Hyperosmolality inhibits exocytosis in sea urchin eggs by formation of a granule-free zone and arrest of pore widening.The Journal of membrane biology · 1989Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We have used the isolated planar cortex of sea urchin eggs to examine the role of osmotic forces in exocytosis by morphological and physiological methods. Electron micrographs of rotary-shadowed replicas show an en face view of exocytosis and demonstrate fusion of cortical vesicles to the underlying oolemma upon addition of calcium. Freeze-fracture replicas of rapidly frozen cortices reveal specialized attachment sites between cortical vesicles and the oolemma, and between the cortical vesicles themselves. We describe a novel light scattering assay for the kinetics of fusion which allows rapid changes of solutions and monitors exocytosis in real time. The rate and extent of fusion are found to be calcium dependent. The removal of calcium halts exocytosis. The validation of exocytosis in this system and development of tools for kinetic analysis allowed us to test predictions of the osmotic hypothesis of exocytosis: hyperosmotic media should inhibit exocytosis; calcium should cause vesicular swelling. Cortical vesicles were found to be permeant to sucrose, glucose, and urea. In media made hyperosmotic with 1.7 M sucrose, cortical vesicles were seen to shrink. Addition of calcium in hyperosmotic media led to a 10-fold decrease in the rate of exocytosis compared with the isotonic rate. The rate, while triggered by calcium, was no longer calcium-dependent. This slowing of exocytosis allowed us to photograph the swelling of cortical vesicles caused by calcium. Removal of calcium had no effect on subsequent exocytosis. Return of cortices to isotonic medium without calcium led to immediate exocytosis. These results are consistent with the idea that swelling of cortical vesicles is required for fusion of biological membranes.
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