CrebA regulation of secretory capacity: Genome-wide transcription profiling coupled with in vivo DNA binding studies.

D J Jackson, D Peng, S A Shinde, A Holenarasipura, P Cahan, D J Andrew

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Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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CrebA regulation of secretory capacity: genome-wide transcription profiling coupled with in vivo DNA binding studies.2025

5 · Who and what money

Authors and funding

6 authors.

D J JacksonEqual first authors.

D PengEqual first authors.

S A ShindeDepartment of Cell Biology, The Johns Hopkins University School of Medicine.

A HolenarasipuraDepartment of Cell Biology, The Johns Hopkins University School of Medicine.

P CahanDepartment of Biomedical Engineering, The Johns Hopkins University School of Medicine.ORCID 0000-0003-3652-2540

D J AndrewDepartment of Cell Biology, The Johns Hopkins University School of Medicine.ORCID 0000-0003-1051-6935

Funding

FORMATION OF THE DROSOPHILA SALIVARY GLANDR01DE013899 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI Deborah J Andrew · 2001 to 2026

$11.2M

NIDCR NIH HHS R01 DE013899

6 · The paper itself

Abstract

DNA binding assays, expression analyses, and binding site mutagenesis revealed that the Drosophila CrebA transcription factor (TF) boosts secretory capacity in the embryonic salivary gland (SG) through direct regulation of secretory pathway component genes (SPCGs). The mammalian orthologues of CrebA, the Creb3L-family of leucine zipper TFs, not only activate SPCG expression in a variety of mammalian tissues but can also activate SPCG expression in Drosophila embryos, suggesting a highly conserved role for this family of proteins in boosting secretory capacity. However, in vivo assays reveal that CrebA binds far more genes than it regulates, and it remains unclear what distinguishes functional binding. It is also unclear if CrebA is the major factor driving SPCG gene expression in all Drosophila embryonic tissues and/or if CrebA also regulates other tissue-specific functions. Thus, we did single cell RNA sequencing (scRNA-seq) of wild-type (WT) and

Indexed as

Chromatin Immunoprecipitation and sequencing (ChIP-Seq)CrebAsalivary glandsecretionSecretory pathway component genes (SPCGs)single cell RNA seq (scRNA-Seq)

Identifiers

PMID40667345

DOI10.1101/2025.06.12.659381

PMCPMC12262295

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