Evidence map›Paper›PMID 40667249›Full record

ArticlebioRxiv : the preprint server for biology2025

Directed evolution of L-DOPA sensing and production enables efficient incorporation of an expanded genetic code into proteins.

Andrew R Gilmour, Qiyao Wei, Chad Wang, Jessica Hellinger, Vidya Pandarinath, Simon d'Oelsnitz, Eli Powell, Jennifer S Brodbelt, Andrew D Ellington, Ross Thyer

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Andrew R GilmourSystems, Synthetic, and Physical Biology, Rice University, Houston, TX.ORCID 0000-0001-8422-2863
Qiyao WeiDepartment of Bioengineering, Rice University, Houston, TX.ORCID 0000-0002-1147-6405
Chad WangDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX.ORCID 0000-0002-8431-3199
Jessica HellingerDepartment of Chemistry, The University of Texas at Austin, Austin, TX.ORCID 0000-0002-3664-7856
Vidya PandarinathDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX.
Simon d'OelsnitzDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX.ORCID 0000-0001-7512-9157
Eli PowellDepartment of Integrative Biology, The University of Texas at Austin, Austin, TX.ORCID 0009-0003-6525-0318
Jennifer S BrodbeltDepartment of Chemistry, The University of Texas at Austin, Austin, TX.ORCID 0000-0003-3207-0217
Andrew D EllingtonDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX.ORCID 0000-0001-6246-5338
Ross ThyerDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, TX.ORCID 0000-0002-0356-5790

Funding

Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological MoleculesR35GM139658 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI BRODBELT, JENNIFER S. · 2021 to 2025
$2.9M
Synthetic biology for controlled releaseR01EB026533 · NIBIB · UNIVERSITY OF TEXAS AT AUSTIN · PI ELLINGTON, ANDREW D · 2019 to 2022
$1.4M
Recombinant antibodies containing L-DOPA for stability, functionalization and selectionK99CA207870 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI THYER, ROSS T · 2016 to 2017
$186k
NCI NIH HHS K99 CA207870NIBIB NIH HHS R01 EB026533NIGMS NIH HHS R35 GM139658
6 · The paper itself

Abstract

3,4-dihydroxyphenylalanine (L-DOPA) is a catechol-containing amino acid derived from L-tyrosine that can be introduced into proteins primarily as a post-translational modification. It can also be introduced in a site-specific manner into proteins via orthogonal tRNA synthetase:tRNA pairs. However, despite the interest in expanded genetic codes containing L-DOPA, efforts to improve metabolism relating to its production have been limited. Here, we report and engineer a LysR-family transcription factor, PP_2251, that can serve as a biosensor for L-DOPA, thereby allowing the optimization of genetic circuits for vastly improved L-DOPA production via compartmentalized partnered replication (CPR), an emulsion PCR-based directed evolution scheme. Using this biosensor, a promiscuous

Identifiers

PMID40667249
PMCPMC12262335

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.