Evidence map›Paper›PMID 40667234›Full record

ArticlebioRxiv : the preprint server for biology2025

Improving Recombinant Antibody Production Using FcBAR: An In Situ Approach to Detect and Amplify Protein-Protein Interactions.

Mina Ying Min Wu, Frances Rocamora, Mojtaba Samoudi, Caressa M Robinson, Chih-Chung Kuo, Nuša Pristovšek, Lise Marie Grav, Helene Faustrup Kildegaard, Gyun Min Lee, Alexandre Rosa Campos and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Mina Ying Min WuDept of Bioengineering, University of California, San Diego.
Frances RocamoraDept of Pediatrics, University of California, San Diego.
Mojtaba SamoudiDept of Pediatrics, University of California, San Diego.
Caressa M RobinsonDept of Bioengineering, University of California, San Diego.
Chih-Chung KuoDept of Bioengineering, University of California, San Diego.
Nuša PristovšekNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark.
Lise Marie GravNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark.
Helene Faustrup KildegaardNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark.
Gyun Min LeeDepartment of Biological Sciences, KAIST.
Alexandre Rosa CamposSanford Burnham Prebys Medical Discovery Institute.
Nathan E LewisDept of Bioengineering, University of California, San Diego.ORCID 0000-0001-7700-3654

Funding

Unraveling the mammalian secretory pathway through systems biology and algorithm developmentR35GM119850 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LEWIS, NATHAN ENOCH · 2016 to 2025
$4.3M
NIGMS NIH HHS R35 GM119850
6 · The paper itself

Abstract

Recombinant proteins, in particular monoclonal antibodies and related molecules, have become dominant therapeutics. As they are produced in mammalian cells, they require the concerted function of hundreds of host cell proteins in the protein secretion pathway. However, the comprehensive set of host cell machinery involved remains unclear. Thus, it is often unknown why some recombinant proteins fail to express well. Here we present and deploy an approach called Fc-targeting Biotinylation by Antibody Recognition (FcBAR), which allows for the

Identifiers

PMID40667234
PMCPMC12262524

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.