Evidence map›Paper›PMID 40667160›Full record

ArticlebioRxiv : the preprint server for biology2025

Rapid acquisition of HIV-1 neutralization breadth in a rhesus V2 apex germline antibody mouse model after a single bolus immunization.

Amrit Raj Ghosh, Rumi Habib, Nitesh Mishra, Ryan S Roark, Madhav Akauliya, Ali A Albowaidey, Joel D Allen, Khaled Amereh, Gabriel Avillion, Maria Bottermann and 35 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

45 authors.

Amrit Raj GhoshRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Rumi HabibDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.ORCID 0000-0002-2812-8263
Nitesh MishraDepartment of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Ryan S RoarkAaron Diamond AIDS Research Center, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Madhav AkauliyaRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Ali A AlbowaideyRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Joel D AllenSchool of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.ORCID 0000-0003-2547-968X
Khaled AmerehDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Gabriel AvillionDepartment of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Maria BottermannRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Bo LiangDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Namit ChaudharyDepartment of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Sean CallaghanDepartment of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Jonathan DyeKoch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.
Xuduo LiDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Jordan R Ellis-PughRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Rohan Roy ChowdhuryDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Nicole E JamesSchool of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.
Xiaotie LiuRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Laura MaiorinoKoch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.
Paula MaldonadoRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Rebecca NedellecDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Prabhgun OberoiDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Kirsten J SowersDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Younghoon ParkDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Thavaleak PrumRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Linette RodriguezDepartment of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Maria SsoziRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Jon TorresDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 28 92037, USA.
Agnes A WalshDepartment of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
John E WarnerRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Stephanie R WeldonRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Liling XuRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Kevin WieheDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Max CrispinSchool of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.ORCID 0000-0002-1072-2694
Andrew B WardConsortium for HIV/AIDS Vaccine Development (CHAVD), The Scripps Research Institute, La Jolla, CA 92037, USA.ORCID 0000-0001-7153-3769
Usha NairRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Beatrice H HahnDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Dennis R BurtonRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Lawrence ShapiroDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10027, USA.
Peter D KwongDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10027, USA.
Darrell J IrvineRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.
Raiees AndrabiDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
George M ShawDepartments of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Facundo D BatistaRagon Institute of Mass General, MIT, and Harvard, Cambridge, MA, 02139, USA.

Funding

B cell lineage-directed rational vaccine strategies based on CAP256SU Env-Ab co-evolutionR01AI167716 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI Raiees Ahmad Andrabi · 2022 to 2026
$4.8M
Combining germline-targeting, B cell immunofocusing and Env-Ab coevolution strategies to induce HIV Envelope V2-apex broadly neutralizing antibodiesR61AI161818 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI ANDRABI, RAIEES AHMAD, SHAW, GEORGE M · 2021 to 2023
$4.7M
Reverse Vaccinology in SHIV Infected Macaques as a Molecular Guide for HIV-1 Vaccine DesignR01AI160607 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI SHAW, GEORGE M · 2021 to 2024
$3.2M
Gates Foundation INV-041767NIAID NIH HHS R01 AI160607NIAID NIH HHS R01 AI167716NIAID NIH HHS R61 AI161818
6 · The paper itself

Abstract

Current vaccine strategies to elicit broadly neutralizing antibodies (bnAbs) against HIV-1 generally propose complex, multi-boost immunization regimens. In rhesus macaques, SHIV infection has been observed to rapidly drive the development of some classes of bnAbs that share structural similarities with those in humans. Here, we generated a knockin mouse model with B cells bearing the unmutated common ancestor (UCA) of the V2 apex-targeted bnAb lineage, V033-a. A single immunization of mice with a germline-targeting native-like trimer was sufficient to recapitulate the ontogeny of the mature rhesus bnAb in knockin mice-including rare, disfavored somatic mutations-leading to the induction of antibodies that exhibited potent neutralization against both autologous and heterologous tier 2 viruses. A boost with Env escape mutant trimers further improved breadth and potency, and cryo-EM structure revealed the structural basis for heterologous neutralization breadth. Non-human primate and mouse models can thus combine with structure to serve as a platform for identifying and confirming immunogens that streamline HIV-vaccination regimens.

Identifiers

PMID40667160
PMCPMC12262380

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