Evidence map›Paper›PMID 40667101›Full record

ArticlebioRxiv : the preprint server for biology2025

Collaboration between two conserved sequence motifs drives ATPase stimulation of Hsp90 by Aha1.

Desmond Prah Amoah, Rebecca Mercier, Gnin Alyousef, Jill L Johnson, Paul LaPointe

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Desmond Prah AmoahDepartment of Cell Biology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7.
Rebecca MercierDepartment of Cell Biology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7.
Gnin AlyousefDepartment of Cell Biology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7.
Jill L JohnsonDepartment of Biological Sciences and the Center for Reproductive Biology, University of Idaho, Moscow, ID, 83844, USA.
Paul LaPointeDepartment of Cell Biology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7.ORCID 0000-0002-5465-1795

Funding

Diversity Supplement: Determinants of Hsp90-client interactionR01GM127675 · NIGMS · UNIVERSITY OF IDAHO · PI JOHNSON, JILL L · 2019 to 2022
$1.2M
NIGMS NIH HHS R01 GM127675
6 · The paper itself

Abstract

Hsp90 is a dimeric molecular chaperone essential for the folding, stabilization, activation, and maturation of hundreds of client proteins, which are critical for cellular function. Co-chaperones, such as Aha1, play a key role in regulating the ATP-dependent Hsp90 client activation cycle by modulating Hsp90's ATPase activity and controlling progression through the cycle. Two highly conserved motifs in Aha1-the NxNNWHW and RKxK motifs-are known to regulate specific aspects of the Hsp90 ATPase cycle. In this study, we demonstrate that the K60 residue within the RKxK motif facilitates the structural organization of the NxNNWHW motif prior to ATP hydrolysis. Mutation of the K60 residue partially impairs the

Identifiers

PMID40667101
PMCPMC12262519

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.