Evidence map›Paper›PMID 40664931›Full record

ArticleScientific reports2025

Detection of tomato brown rugose fruit virus through CRISPR-Cas12a and CRISPR-Cas9 systems.

Masoud Besati, Mohammad Reza Safarnejad, Atousa Aliahmadi, Mohsen Farzaneh, Raúl Ruiz, Roser Montagud-Martínez, Guillermo Rodrigo, Hasan Rafati

Abstract read
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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Masoud BesatiDepartment of Pharmaceutical Engineering, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran.
Mohammad Reza SafarnejadIranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran. mrsafarnejad@yahoo.com.
Atousa AliahmadiDepartment of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
Mohsen FarzanehDepartment of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.
Raúl RuizInstitute for Integrative Systems Biology (I2SysBio), CSIC- University of Valencia, Paterna, Spain.
Roser Montagud-MartínezInstitute for Integrative Systems Biology (I2SysBio), CSIC- University of Valencia, Paterna, Spain.
Guillermo RodrigoInstitute for Integrative Systems Biology (I2SysBio), CSIC- University of Valencia, Paterna, Spain.
Hasan RafatiDepartment of Pharmaceutical Engineering, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran. H_Rafati@sbu.ac.ir.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tomato brown rugose fruit virus (ToBRFV) is a single-stranded positive-sense RNA virus that targets tomato and pepper plants and is causing significant damage to crops in some regions of the world. ToBRFV is a highly contagious virus that is stable and rapidly spreads by mechanical methods and seeds. As a result, it may spread both locally and over long distances, and it is now recognized as a pandemic in plants. This study investigates the effectiveness of the systems CRISPR-Cas12a and CRISPR-Cas9, in conjugation with recombinase polymerase amplification (RPA), to detect ToBRFV in tomato plant samples collected from the field. The trans-cleavage activity of both nucleases, Cas12a and Cas9, was exploited to process a probe labelled with fluorescein and biotin to be resolved on a lateral flow device, thereby enabling a visual readout. We were able to detect the RNA genome of the virus in about 1 h at a low constant temperature. These results could pave way to offer a rapid, sensitive, and specific method for on-site detection of ToBRFV.

Indexed as

CRISPR-Cas SystemsPlant DiseasesSolanum lycopersicumBacterial ProteinsCRISPR-Associated ProteinsEndodeoxyribonucleasesBacterial ProteinsCas12a proteinCRISPR-Associated ProteinsEndodeoxyribonucleasesCRISPR diagnosticsLateral flow assayPlant virus

Identifiers

PMID40664931
PMCPMC12264260

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.