ArticleScientific reports2025
Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Carcass-Based Surveillance of Amphibian Herpesviruses, Ranaviruses andPathogens (Basel, Switzerland) · 2026Article
- Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study assesses the feasibility of extracting high-quality DNA from blood samples stored at - 20 °C for up to 21 years under suboptimal conditions. It addresses sample mishandling in research, where many samples lack proper biobank protocols. Prior studies focused on short-term storage and controlled conditions, highlighting the negative effects of freeze-thaw cycles. This study evaluates whether DNA from long-term stored samples under suboptimal conditions can still meet quality standards for research purposes. Genomic DNA was extracted from 1012 capillary blood samples from the Diabetes Prediction in Skåne study. Samples were stored at - 20 °C for 7-21 years, and DNA was isolated using QIAamp DNA Blood Mini kits. DNA quantity, purity, and quality were analysed using spectrophotometry and automated electrophoresis. Overall, 75.7% of samples met quality standards for DNA quantity (≥ 20 ng/µL) and purity (A260/280 ratio 1.7-1.9), with the highest proportion in 12-year samples (83.5%). DNA quality was further assessed in 270 samples, where 57.8% had a DNA Integrity Number (DIN) of 7 or higher. This study suggests that historical blood samples stored under suboptmal conditions can still be viable for modern genomic analyses.
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