ArticleMethods in molecular biology (Clifton, N.J.)2025
The Construction of dsDNA-Based AND-Gate (DBAG) Peptide Library in Mammalian Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The construction of DNA-encoded peptide libraries in mammalian cells is an evolving field that combines the advantages of mammalian cell expression systems with the vast diversity of DNA-encoded libraries. This enables high-throughput screening and identification of functional peptides in a mammalian cell context, aiding in the elucidation of disease-related proteins and the development of novel peptide drugs. We have developed a strategy for constructing dsDNA-based AND-gate (DBAG), which utilizes dsDNAs with NNK degenerate codons at the ends to implement AND-gate genetic circuits and generate peptide libraries in mammalian cells. We provide a detailed description of the library preparation, transfection, and analysis methods.
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