ArticleMethods in molecular biology (Clifton, N.J.)2025
Quantitative Affinity Screening of Macrocyclic Peptide Libraries Using Yeast Surface Display Technology.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Yeast Display Technology Enables Rapid Discovery of Low-Nanomolar Macrocyclic Peptide Inhibitors of Human Angiotensin-Converting Enzyme 2.Journal of medicinal chemistry · 2026Article
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Authors and funding
7 authors.
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Abstract
This protocol details the use of yeast surface display technology for the in vitro-directed evolution of disulfide-tethered macrocyclic peptide ligands. In the first section, we describe the generation of large naïve combinatorial libraries encoding cysteine-rich peptide sequences expressed on the yeast cell surface using homologous recombination-based methods. In the second section, we detail the use of fluorescence-activated cell sorting to rapidly and effectively isolate yeast-encoding disulfide-tethered macrocyclic peptide ligands with favorable binding properties. In the last section, we describe the quantitative characterization of isolated disulfide-tethered macrocyclic peptide ligand variants directly as yeast cell surface fusions, thus eliminating the need for costly and time-consuming synthesis and purification.
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