Evidence map›Paper›PMID 40663331›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Facile High-Throughput Pre-purification of Biological Samples for Library-Scale Peptide Interaction Studies.

Ulrike Beutling, Sven-Kevin Hotop, Ronald Frank, Mark Brönstrup

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ulrike BeutlingDepartment of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Sven-Kevin HotopDepartment of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Ronald FrankAIMS Scientific Products GmbH, Berlin, Germany.
Mark BrönstrupDepartment of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany. Mark.Broenstrup@helmholtz-hzi.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Peptides and peptide libraries are valuable probes in solid-phase binding assays and widely used in studies of protein-protein interactions. However, a high unspecific background can disturb or even inhibit a successful experiment. We describe an easy and flexible method for pre-purification of samples that is particularly well-suited for high-throughput processing of large numbers of samples.

Indexed as

High-Throughput Screening AssaysPeptide LibraryPeptidesProtein Interaction MappingProteinsHumansProtein BindingPeptide LibraryPeptidesProteinsBackground eliminationPeptide librariesSolid-phase binding assay

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.