Evidence map›Paper›PMID 40663326›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Protease Specificity Profiling Using Synthetic Combinatorial Peptide Libraries and Mass Spectrometry.

Bart Claushuis, Robert A Cordfunke, Arnoud H de Ru, Peter A van Veelen, Jeroen Corver, Jan W Drijfhout, Paul J Hensbergen

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Bart ClaushuisCenter for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Robert A CordfunkeDepartment of Immunology, Leiden University Medical Center, Leiden, The Netherlands.
Arnoud H de RuCenter for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Peter A van VeelenCenter for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Jeroen CorverLeiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
Jan W DrijfhoutDepartment of Immunology, Leiden University Medical Center, Leiden, The Netherlands.
Paul J HensbergenCenter for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands. P.J.Hensbergen@lumc.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The substrate specificity of proteases largely determines their function and biological role. Methods that can profile a protease's specificity with high detail are valuable tools for protease research. Synthetic combinatorial peptide libraries offer a large array of potential substrates that can be tested in a single experiment. Here, we describe the design and synthesis of a combinatorial peptide library using the one-bead-one-compound approach, followed by treatment with a protease, i.e., Pro-Pro endopeptidase (PPEP), and subsequent LC-MS/MS and data analysis.

Indexed as

Combinatorial Chemistry TechniquesPeptide HydrolasesPeptide LibraryTandem Mass SpectrometryChromatography, LiquidPeptidesSubstrate SpecificityPeptide HydrolasesPeptide LibraryPeptidesBacterial adhesionLC-MS/MSMetalloproteasePPEPPro-Pro endopeptidaseProtease

Identifiers

PMID40663326

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.