Efficient Detection of N-sialoglycans in N-glycomics Using Mass Spectrometry by nMatch.

Shanshan Zhao, Shouwen Du, Yudong Guan, Hartmut Schlüter

Abstract read

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Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Shanshan ZhaoDepartment of Critical Care Medicine, Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People's Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China.

Shouwen DuKey Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs; Key Laboratory of Livestock Disease Prevention of Guangdong Province, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, Guangdong, China.

Yudong GuanDepartment of Critical Care Medicine, Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People's Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China. guan181992@163.com.

Hartmut SchlüterSection Mass Spectrometry and Proteomics, Center for Diagnostics, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany. hschluet@uke.de.

Funding

China Scholarship Council 202308080031High-level talents of Zhongzhou laboratory 2024TZ0001National Natural Science Foundation of China 32101189

6 · The paper itself

Abstract

The structural instability and electronegativity of sialic acids make N-sialoglycan profiling challenging. We compared commonly used preparation strategies, including native, acetohydrazide-based derivatization, and permethylation, in N-glycomics with nMatch, an R-based bioinformatics tool that ensures full coverage of the detected N-glycans using mass spectrometry. We demonstrated that permethylation leads to efficient detection of not only N-sialoglycans but also other N-glycans and, therefore, provides a valuable reference for the selection of the appropriate N-glycan preparation strategy in future studies.

Indexed as

GlycomicsMass SpectrometryPolysaccharidesSialic AcidsPolysaccharidesSialic AcidsMass spectrometryN-glycomicsnMatchN-sialoglycans

Identifiers

PMID40663308

DOI10.1038/srep46206

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.