Evidence map›Paper›PMID 40663269›Full record

ArticleMolecular neurobiology2025

Integrative Single-Cell Analysis Decodes Gene Expression and Chromatin Accessibility in the Developing Human Fetal Brain.

Tiantian Xu, Huihui Tao, Lingling Zhou, Haiyan Yu, Shi Bai, Hui Guo, Zhipeng Zeng, Wei Shi, Chunmei Wen, Mengyao Wu and 6 more

Abstract read
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In one paragraph

Article in Molecular neurobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. ELF5-Mediated Enhancer-Promoter Interaction Regulates LALBA Expression in Ovine Mammary Gland.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Tiantian Xu *School of Medicine, Anhui University of Science & Technology, Huainan, China.
Huihui Tao *School of Medicine, Anhui University of Science & Technology, Huainan, China. 475433093@qq.com.
Lingling ZhouSchool of Medicine, Anhui University of Science & Technology, Huainan, China.
Haiyan YuPingshan District Peoples' Hospital of Shenzhen, Shenzhen, Guangdong, P.R. China.
Shi BaiDepartment of Anatomy, School of Medicine, Taizhou University, Jiaojiang, China.
Hui GuoLaboratory of Genetic Disease Diagnosis and Prenatal Diagnosis Center, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, China.
Zhipeng ZengLaboratory of Genetic Disease Diagnosis and Prenatal Diagnosis Center, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, China.
Wei ShiDepartment of Obstetrics, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, China.
Chunmei WenSchool of Medicine, Anhui University of Science & Technology, Huainan, China.
Mengyao WuSchool of Medicine, Anhui University of Science & Technology, Huainan, China.
Xuejia ZhengThe first Hospital, Anhui University of Science & Technology, Huainan, China.
Pingping YeDepartment of Obstetrics, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, China.
Yuan FangDepartment of Obstetrics, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, China.
Mingquan GuoSchool of Medicine, Anhui University of Science & Technology, Huainan, China.
Donge TangLaboratory of Genetic Disease Diagnosis and Prenatal Diagnosis Center, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, China. donge66@126.com.
Yong DaiSchool of Medicine, Anhui University of Science & Technology, Huainan, China. daiyong22@aust.edu.cn.

Funding

Shenzhen Science and Technology Program NO.JCYJ20220530152015035Taizhou Science and Technology Bureau NO.24ywa51
6 · The paper itself

Abstract

The brain is the core of the central nervous system, responsible for regulating and integrating various physiological and psychological functions. Abnormal disruptions in genes during brain development can lead to a range of neurodevelopmental disorders. In this study, we performed a systematic investigation of human fetal brain tissue from miscarriages between 8 and 17 weeks of gestation using integrated single-cell RNA sequencing (scRNA-seq) and single-cell transposase-accessible chromatin sequencing (scATAC-seq). We constructed single-cell transcriptomic and epigenomic maps of neurodevelopment, revealing key signaling pathways involved in neural cell proliferation, differentiation, and functional maturation. Through pseudotime analysis, we reconstructed the developmental trajectory of neuronal differentiation and its dynamic regulatory mechanisms. Additionally, we identified cell type-specific chromatin accessibility regions during neurogenesis and, through integrated analysis, predicted potential regulatory elements involved in the process. Overall, the single-cell multi-omics integration map constructed in this study provides valuable resources for a deeper understanding of fetal brain development, cellular heterogeneity, lineage relationships, and transcriptional regulatory networks during neurogenesis.

Indexed as

BrainChromatinFetusGene Expression Regulation, DevelopmentalSingle-Cell AnalysisFemaleGene Regulatory NetworksHumansNeurogenesisTranscriptomeChromatinDevelopmentHuman fetal brainOmicsSingle-cell ATAC-seqSingle-cell RNA-seq

Identifiers

PMID40663269

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.