Evidence map›Paper›PMID 40663185›Full record

ArticleMolecular biology reports2025

Differential gene expression in the intestines of Chinese mitten crabs with three carapace colors.

Qi Zhu, Maolei Wei, Xinxin Chen, Zhichao Rong, Dongran Yang, Xiaowu Chen, Xugan Wu

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Qi Zhu *Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China.
Maolei Wei *Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China.
Xinxin ChenCentre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China.
Zhichao RongCentre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China.
Dongran YangCentre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China.
Xiaowu ChenCentre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China. xwchen@shou.edu.cn.
Xugan WuCentre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China. xgwu@shou.edu.cn.

Funding

The agriculture R&D project from Shanghai Municipal Science and Technology Commission 23N71900100
6 · The paper itself

Abstract

backgroundCarapace color in Chinese mitten crab (Eriocheir sinensis) influences both commercial value and physiological traits, yet its molecular basis remains unclear. The intestine plays a key role in pigment absorption and metabolism, but its involvement in pigmentation has been understudied. This study aimed to investigate intestinal gene expression differences among red, white, and green carapace strains. METHODS AND

resultsWe sequenced intestinal RNA from red, white, and green morphs (n = 3 per group) on an Illumina NovaSeq platform. Following fastp quality control and Salmon quantification, 6,214 genes were identified as differentially expressed (|log2FC| ≥ 1, FDR < 0.05). Functional enrichment linked these genes to pigment transport and biosynthesis pathways, including ABC transporters, SLC carriers, carotenoid binding, and tyrosine metabolism. Seven representative DEGs (CRCN‑A1, CRCN-A2, ABCC4, ABCF1, slc23a1, slc49a4, TYROB) were validated by qRT‑PCR, with expression trends congruent to RNA‑seq results.

conclusionIntestinal gene expression contributes to carapace pigmentation through distinct carotenoid‑transport and melanin pathways. These insights offer molecular targets for selective breeding and functional validation.

Indexed as

BrachyuraPigmentationAnimalsAnimal ShellsColorGene Expression ProfilingGene Expression RegulationIntestinesTranscriptomeCarapace pigmentationCarotenoidsEriocheir sinensisMelaninTranscriptome

Identifiers

PMID40663185

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