Evidence map›Paper›PMID 40662559›Full record

ArticleProtein and peptide letters2025

Purification, Crystallization, and Preliminary X-ray Diffraction Studies on Hemoglobin from the Angora Goat (

Farheen Pervez, Shobana Nagaraj, Mpho Setshedi, Arshad Mather, Ponnuswamy Mondikalipudur Nanjappa Gounder, Yasien Sayed, Ramesh Pandian

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Article in Protein and peptide letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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7 authors.

Farheen PervezProtein Structure-Function Research Laboratory, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.ORCID 0009-0002-4028-0580
Shobana NagarajChromium Research Group, Department of Physics, University of Johannesburg, Auckland Park, Johannesburg 2006, South Africa.ORCID 0000-0003-1220-2999
Mpho SetshediProtein Structure-Function Research Laboratory, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.ORCID 0000-0001-5117-4092
Arshad MatherAgricultural Research Council - Onderstepoort Veterinary Research, Private Bag X5, Onderstepoort, Pretoria 0110, South Africa.ORCID 0000-0001-7455-2738
Ponnuswamy Mondikalipudur Nanjappa GounderCentre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.ORCID 0000-0001-8871-1082
Yasien SayedProtein Structure-Function Research Laboratory, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.ORCID 0000-0002-1781-2115
Ramesh PandianProtein Structure-Function Research Laboratory, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.ORCID 0000-0002-4947-7243

Funding

National Equipment Program 129920National Research Foundation of South Africa RPND023, RPAN023
6 · The paper itself

Abstract

introductionAngora goats are a distinct breed that differs significantly from common goats and shares a similar appearance to sheep. In Angora goats, only the level of glutathione (GSH) is elevated during under-stimulated conditions, as well as after the period of hypoxic stress; however, no changes are found in 2,3-diphosphoglycerate (2,3-DPG) levels, which are commonly present in the red blood cells (RBCs) of most mammals. We chose the Angora goat for our investigation because no previous studies have been conducted on the structural and functional aspects of hemoglobin (Hb). In addition, no sequence or structural information is currently available in any database.

methodsAngora goat Hb was isolated and purified by anion-exchange chromatography, followed by crystallization using various methods. X-ray data collection for Angora goat Hb was performed under a liquid nitrogen cryo-stream using a

resultsGood diffracting crystals were obtained using the hanging-drop vapor-diffusion method with polyethylene glycol (PEG) 3350 as the precipitant in water, without the addition of any salt or buffer. The Angora goat Hb diffracted to a resolution of 1.85 Å, and the structure solution was obtained by the molecular replacement method, using the structure of domestic goat Hb as the starting model. DISCUSSION: The solved structure of Angora goat crystallized in the monoclinic space group P21, consisting of one whole biological molecule in the asymmetric unit, with unit cell dimensions of a = 52.08 Å, b = 76.70 Å, c = 74.08 Å, and β = 91.77 °. The solvent content and Matthews coefficient (Vm) for the Angora goat Hb are 49.05% and 2.41 Å

conclusionPurification, crystallization, and preliminary X-ray diffraction studies of Angora goat Hb were performed successfully. Structural refinement and biophysical characterization of Angora goat Hb are in progress in the absence and presence of GSH and 2,3-DPG.

Indexed as

GoatsHemoglobinsAnimalsCrystallizationCrystallography, X-RayX-Ray DiffractionHemoglobinsAngora goathemoglobinhypoxic stresslevel of glutathioneoxygen affinityprotein crystals.

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PMID40662559

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