Identification of DNA Replication Stress-Related Genes as Prognostic Biomarkers for Bladder Cancer.

Fei Zhang, Shuai Li, Zhijie Zhang, Jiulong Li, Huiqin Liu, Xudong Ma, Zhigang Yang

Abstract read

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Article in Combinatorial chemistry & high throughput screening, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Fei ZhangDepartment of Urology, Baotou Central Hospital, Inner Mongolia, Baotou, 014040, China.

Shuai LiDepartment of Urology, Baotou Central Hospital, Inner Mongolia, Baotou, 014040, China.

Zhijie ZhangDepartment of Obstetrics and Gynecology, Baotou Central Hospital, Inner Mongolia, Baotou, 014040, China.

Jiulong LiBaotou Central Clinical Medical College, Inner Mongolia Medical University, Inner Mongolia, Baotou 014040, China.

Huiqin LiuDepartment of Obstetrics and Gynecology, Baotou Central Hospital, Inner Mongolia, Baotou, 014040, China.ORCID 0009-0006-5167-2458

Xudong MaDepartment of Urology, Baotou Central Hospital, Inner Mongolia, Baotou, 014040, China.ORCID 0000-0001-5644-7221

Zhigang YangDepartment of Urology, Baotou Central Hospital, Inner Mongolia, Baotou, 014040, China.ORCID 0009-0009-5673-4357

Funding

Joint Research Fund of Public Hospitals, Inner Mongolia Academy of Medical Sciences 2023GLLH0234

6 · The paper itself

Abstract

introductionBladder cancer (BLCA) is a highly aggressive malignancy with poor prognosis. DNA replication stress-related genes (DRSGs) hold prognostic significance in multiple cancers, and their expression patterns in BLCA may reveal novel biomarkers and therapeutic targets.

methodsThis study was designed using a public database and the Cancer Genome Atlas (TCGA). Genes associated with DNA replication stress in BLCA were discovered by analyzing data from the TCGA and GEO databases using bioinformatics tools. The prognostic gene expression profiles in BLCA cell lines were analyzed using Western blotting (WB). The motility capacity of BLCA cells was evaluated using the wound healing and Transwell migration assays, while cell growth was ascertained with the CCK-8 assay.

resultsFive DRSGs with prognostic significance were identified, and a risk score model was constructed using univariate Cox regression and the Least Absolute Shrinkage and Selection Operator (LASSO) regression algorithm. Kaplan-Meier (KM) analysis showed worse Overall Survival (OS) in the high-risk group (P < 0.05). Gene Set Enrichment Analysis (GSEA) indicated involvement in tumor-related pathways. The nomogram effectively predicted OS in both training and validation cohorts. WB and functional assays confirmed gene expression and effects on BLCA cell proliferation and migration. DISCUSSION: This study first validates DRSGs' prognostic value in bladder cancer, highlighting potential biomarkers and targets. Limitations include reliance on public data and

Indexed as

Biomarkers, TumorDNA ReplicationUrinary Bladder NeoplasmsCell Line, TumorCell MovementCell ProliferationHumansPrognosisBiomarkers, TumorbioinformaticsBladder cancerDNA replication stressimmune infiltrationprognosistherapeutic targets

Identifiers

PMID40662552

DOI10.1016/j.isci.2023.107703

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