ArticleVeterinary clinical pathology2025
Method Comparison and Investigation of Interference Variables of a Hand-Held Hemoglobinometer(HemoCue Hb 201
Article in Veterinary clinical pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundRegular blood sampling to monitor RBC mass in anemic cats can exacerbate anemia. Laboratory-based reporting can delay clinical decisions. A hand-held hemoglobinometer, HemoCue Hb 201
objectivesThis preliminary study aimed to evaluate the utility of HC-201 in cats and investigate the impact of potential interferents on its performance.
methodsOne hundred and fifty-four venous blood samples in EDTA from 93 cats were analyzed. Hemoglobin concentration was measured once using an ADVIA 2120 analyzer and compared to the mean of 2-3 replicate measurements from an HC-201. Agreement and systematic bias between HC-201 and ADVIA results, along with precision between HC-201 replicates, were assessed using Lin's concordance correlation coefficient, non-parametric Bland-Altman, Passing-Bablok regression, and intraclass correlation coefficient. The performance of HC-201 in the presence of anemia, leukocytosis, azotemia, lipemia, icterus, hemolysis, and peripheral versus jugular venipunctures was assessed using Wilcoxon rank-sum tests.
resultsPassing-Bablok analysis revealed a significant constant bias (intercept = -2.242, 95% CI: -4.042 to -0.667) but no significant proportional bias (slope = 1.015, 95% CI: 1.000-1.032). HC-201 demonstrated excellent agreement (ρ
conclusionsThis preliminary study indicates that HC-201 offers reliable point-of-care monitoring for hemoglobin concentration in cats.
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