Evidence map›Paper›PMID 40661628›Full record

ArticlebioRxiv : the preprint server for biology2025

Purification of post-transcriptionally modified tRNAs for enhanced cell-free translation systems.

Evan M Kalb, Jose L Alejo, Leticia Dias-Fields, Isaac Knudson, Joshua A Davisson, Efren Maldonado, Kanokporn Chattrakun, Shangsi Lin, Alanna Schepartz, Shenglong Zhang and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Evan M KalbDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN US.
Jose L AlejoDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN US.
Leticia Dias-FieldsDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN US.
Isaac KnudsonDepartment of Chemistry, University of California, Berkeley, CA, USA.
Joshua A DavissonDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN US.
Efren MaldonadoDepartment of Structural Biology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Kanokporn ChattrakunDepartment of Structural Biology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Shangsi LinDepartment of Chemistry and The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
Alanna SchepartzDepartment of Chemistry, University of California, Berkeley, CA, USA.
Shenglong ZhangDepartment of Chemistry and The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
Scott BlanchardDepartment of Structural Biology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Aaron E EngelhartDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN US.
Katarzyna P AdamalaDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN US.

Funding

Center for Multi-Scale Analysis of the Human EpitranscriptomeRM1HG011563 · NHGRI · WEILL MEDICAL COLL OF CORNELL UNIV · PI SAMIE R JAFFREY, Kathryn D Meyer · 2021 to 2026
$22.2M
Technology Development Coordinating CenterU24HG011735 · NHGRI · JACKSON LABORATORY · PI Mark D ADAMS · 2021 to 2026
$8.1M
Imaging Protein Synthesis on the Ribosome using Single-Molecule FRETR01GM079238 · NIGMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI BLANCHARD, SCOTT C · 2006 to 2023
$7.0M
Development of Next-Generation Mass Spectrometry-based de novo RNA Sequencing for all ModificationsR01HG012853 · NHGRI · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Shenglong Zhang · 2023 to 2026
$2.6M
Development of new tags for RNA visualizationR01GM152459 · NIGMS · UNIVERSITY OF MINNESOTA · PI Aaron E. Engelhart · 2024 to 2026
$1.1M
High-throughput Direct Sequencing and Quantitative Mapping of RNA Modifications using Mass Spectrometry.R41HG013624 · NHGRI · DIRECTSEQ BIOSCIENCES, INC. · PI ZHANG, SHENGLONG · 2024 to 2024
$407k
Exhaustive de novo sequencing of every RNA in a sample by a layer-by-layer mass spectrometry ladder intensity approach.R41HG014125 · NHGRI · DIRECTSEQ BIOSCIENCES, INC. · PI ZHANG, SHENGLONG · 2024 to 2024
$215k
NHGRI NIH HHS R01 HG012853NHGRI NIH HHS R41 HG013624NHGRI NIH HHS R41 HG014125NHGRI NIH HHS RM1 HG011563NHGRI NIH HHS U24 HG011735NIGMS NIH HHS R01 GM079238NIGMS NIH HHS R01 GM152459
6 · The paper itself

Abstract

Transfer RNAs (tRNAs) are utilized by the ribosome to decode the nucleic acid alphabet. tRNA structure, stability, aminoacylation efficiency, and decoding efficacy are governed by their extensive post-transcriptional modifications. In most studies, individual tRNAs are generated using

Identifiers

PMID40661628
PMCPMC12259135

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.