Evidence map›Paper›PMID 40661551›Full record

ArticlebioRxiv : the preprint server for biology2025

Intelectin-2 is a broad-spectrum antimicrobial lectin.

Amanda E Dugan, Deepsing Syangtan, Eric B Nonnecke, Rajeev S Chorghade, Amanda L Peiffer, Jenny J Yao, Jessie Ille-Bunn, Dallis Sergio, Gleb Pishchany, Catherine Dhennezel and 13 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors.

Amanda E DuganDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0003-3135-8155
Deepsing SyangtanDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0001-5245-4782
Eric B NonneckeDepartment of Microbiology and Immunology, School of Medicine, University of California-Davis; Davis, CA, USA.ORCID 0000-0001-5345-2009
Rajeev S ChorghadeDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0001-7162-1419
Amanda L PeifferDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0001-8113-9458
Jenny J YaoDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0003-3405-9943
Jessie Ille-BunnDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.
Dallis SergioThe Broad Institute of MIT and Harvard; Cambridge, MA, USA.ORCID 0000-0001-9290-7088
Gleb PishchanyThe Broad Institute of MIT and Harvard; Cambridge, MA, USA.ORCID 0000-0001-9303-010X
Catherine DhennezelThe Broad Institute of MIT and Harvard; Cambridge, MA, USA.ORCID 0000-0003-3273-7165
Hera VlamakisThe Broad Institute of MIT and Harvard; Cambridge, MA, USA.
Sunhee BaeDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0002-8913-9476
Sheila JohnsonDepartment of Biochemistry, University of Wisconsin-Madison; Madison, WI, USA.ORCID 0000-0001-5062-7934
Chariesse EllisDepartment of Biochemistry, University of Wisconsin-Madison; Madison, WI, USA.
Soumi GhoshDepartment of Biology, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0001-6101-0147
Jill W AltyDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0002-9780-1343
Carolyn E BarnesDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.
Miri KrupkinDepartment of Biological Engineering, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0002-5917-0894
Gerardo Cárcamo-OyarceDepartment of Biological Engineering, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0009-0004-4827-4892
Katharina RibbeckDepartment of Biological Engineering, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0001-8260-338X
Ramnik J XavierThe Broad Institute of MIT and Harvard; Cambridge, MA, USA.ORCID 0000-0002-5630-5167
Charles L BevinsDepartment of Microbiology and Immunology, School of Medicine, University of California-Davis; Davis, CA, USA.ORCID 0000-0003-2725-2622
Laura L KiesslingDepartment of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, USA.ORCID 0000-0001-6829-1500

Funding

Pilot & Feasibility ProgramP30DK043351 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI RAYMOND T CHUNG · 1991 to 2026
$35.3M
Synthetic Ligands for Modulating Immune Cell ResponsesR01AI055258 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI KIESSLING, LAURA L · 2003 to 2024
$7.8M
Novel Defensins in Human Epithelial TissuesR37AI032738 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI BEVINS, CHARLES L · 2015 to 2022
$3.1M
Development of multifunctional probes for profiling microbial glycansU01CA231079 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI IMPERIALI, BARBARA, KIESSLING, LAURA L · 2018 to 2020
$1.4M
NCI NIH HHS U01 CA231079NIAID NIH HHS R01 AI055258NIAID NIH HHS R37 AI032738NIDDK NIH HHS P30 DK043351
6 · The paper itself

Abstract

Mammals regulate the localization, composition, and activity of their native microbiota at colonization sites. Lectins residing at these sites influence microbial populations, but their individual functions are often unclear. Intelectins are found in chordates at mucosal barriers, but their functions are not well characterized. We found that mouse intelectin-2 (mItln2) and human intelectin-2 (hItln2) engage and crosslink mucins via carbohydrate recognition. Moreover, both lectins recognize microbes within native microbial communities, including gram-positive and gram-negative isolates from the respiratory and gastrointestinal tracts. This ability to engage mammalian and microbial glycans arises from calcium-coordinated binding of carbohydrate residues within mucus and microbial surfaces. Microbes, but not human cells, bound by mItln2 or hItln2, suffer a loss of viability. These findings underscore the crucial antimicrobial role of mammalian intelectin-2 in mucosal defense, where it plays offensive (microbial killing) and defensive (mucus crosslinking) roles in regulating microbial colonization.

Identifiers

PMID40661551
PMCPMC12259049

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.