Evidence map›Paper›PMID 40661511›Full record

ArticlebioRxiv : the preprint server for biology2025

An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function.

Zeinab Jame-Chenarboo, Edward N Schmidt, Madeline Crichton, Kei Takahashi-Yamashiro, Guilherme M Lima, Liany Luna-Dulcey, Jaesoo Jung, Sabine Ivison, Chris D St Laurent, Jhon R Enterina and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Zeinab Jame-ChenarbooDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Edward N SchmidtDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Madeline CrichtonDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Kei Takahashi-YamashiroDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Guilherme M LimaDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Liany Luna-DulceyDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Jaesoo JungDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Sabine IvisonDepartment of Surgery and BC Children's Hospital Research Institute, University of British Columbia, Canada.
Chris D St LaurentDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Jhon R EnterinaDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Sung-Yao LinDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Susmita SarkarDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Reni JohnDepartment of Pathobiology, College of Veterinary Medicine, University of Illinois, USA.
Som G NanjappaDepartment of Pathobiology, College of Veterinary Medicine, University of Illinois, USA.ORCID 0000-0001-8022-1353
Stacy A MalakerDepartment of Chemistry, Yale University, USA.ORCID 0000-0003-2382-5067
Megan K LevingsDepartment of Surgery and BC Children's Hospital Research Institute, University of British Columbia, Canada.ORCID 0000-0002-0305-5790
Jamey D MarthSanford Burnham Prebys Medical Discovery Institute, La Jolla, USA.
Ratmir DerdaDepartment of Chemistry, University of Alberta, Edmonton, Canada.
Matthew S MacauleyDepartment of Chemistry, University of Alberta, Edmonton, Canada.ORCID 0000-0003-4579-1048

Funding

Protein Glycosylation in the Coagulopathy and Inflammation of SepsisP01HL131474 · NHLBI · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI ESKO, JEFFREY D · 2016 to 2025
$26.2M
GLYCOSYLTRANSFERASE FUNCTION IN DEVELOPMENT AND DISEASER01DK048247 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JAMEY MARTH · 1994 to 2026
$9.4M
Protein Sialylation and De-Sialyation in Cell Surface Glycoprotein Homeostasis and DiseaseR01AI151371 · NIAID · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI MARTH, JAMEY · 2021 to 2025
$2.4M
Regulation of Blood Glycoproteins by Lectin Receptors in Health and DiseaseR01HL158677 · NHLBI · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI JAMEY MARTH · 2023 to 2026
$1.9M
NHLBI NIH HHS P01 HL131474NHLBI NIH HHS R01 HL158677NIAID NIH HHS R01 AI151371NIDDK NIH HHS R01 DK048247
6 · The paper itself

Abstract

Siglecs are immunomodulatory receptors that regulate immune cell function. A fundamental challenge in studying Siglec-ligand interactions is the low affinity of Siglecs for their ligands. Inspired by how nature uses multivalency, we developed Siglec-liposomes as a highly multivalent and versatile platform for detecting Siglec glycan ligands in which recombinant Siglecs were conjugated to liposomes using the SpyCatcher-SpyTag system. Siglec-liposomes offer tunable multivalency and a modular assembly, enabling presentation of different Siglecs on the same liposome. Using Siglec-liposomes, we profiled Siglec ligands on human leukocytes, revealing new insights into Siglec ligands. Moreover, Siglec-liposomes are

Identifiers

PMID40661511
PMCPMC12259067

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.