Evidence map›Paper›PMID 40661429›Full record

ArticlebioRxiv : the preprint server for biology2025

Ensemble quantitation of absolute metabolite concentrations in T cells reveals conserved features of immunometabolism.

Samantha O'Keeffe, Harin Sim, Elle McCue, Junyoung O Park

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Samantha O'KeeffeDepartment of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0009-0009-8884-6708
Harin SimDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0009-0006-3336-1920
Elle McCueDepartment of Mechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0009-0004-4484-9003
Junyoung O ParkDepartment of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0001-9869-8993

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Damoiseaux · 1985 to 2026
$134.5M
Virology CoreP30AI028697 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZACK, JEROME A. · 1991 to 2018
$39.9M
Molecular basis linking metabolic heterogeneity, compatibility, and adaptabilityR35GM143127 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Junyoung O. Park · 2021 to 2026
$2.5M
Quantitative Mass Spectrometer for Targeted and Global Proteomics, MetabolomicsS10OD016387 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GRAEBER, THOMAS G · 2013 to 2013
$593k
Exploring the mechanisms underlying plant (poly)phenols bioactivity via integrating metabolomics and fluxomicsR21AT012814 · NCCIH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PARK, JUNYOUNG O. · 2024 to 2025
$403k
NCCIH NIH HHS R21 AT012814NCI NIH HHS P30 CA016042NIAID NIH HHS P30 AI028697NIGMS NIH HHS R35 GM143127NIH HHS S10 OD016387
6 · The paper itself

Abstract

Cells regulate metabolite levels to efficiently utilize metabolic networks and avoid toxic buildup. In turn, metabolites dictate metabolic activity by acting as substrates, products, and effectors. Despite their foundational role in cell physiology, kinetics, and thermodynamics, absolute metabolite concentrations are seldom known. Here we develop an ensemble method for absolute metabolite quantitation and quantify 84 metabolites in T cells. Liquid chromatography-mass spectrometry of metabolites co-extracted from T cells and

Identifiers

PMID40661429
PMCPMC12259047

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.