Evidence map›Paper›PMID 40661357›Full record

ArticlebioRxiv : the preprint server for biology2025

Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci.

William S Henriques, Jarrett Bowman, Laina N Hall, Colin C Gauvin, Hui Wei, Huihui Kuang, Christina M Zimanyi, Edward T Eng, Andrew Santiago-Frangos, Blake Wiedenheft

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

William S HenriquesDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.ORCID 0000-0001-9836-3827
Jarrett BowmanDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.
Laina N HallDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.ORCID 0000-0003-3135-332X
Colin C GauvinDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA.ORCID 0000-0001-7171-552X
Hui WeiSimons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.ORCID 0000-0001-6777-1772
Huihui KuangSimons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.ORCID 0000-0002-9891-1747
Christina M ZimanyiSimons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.ORCID 0000-0002-6782-507X
Edward T EngSimons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.ORCID 0000-0002-8014-7269
Andrew Santiago-FrangosDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.ORCID 0000-0001-9615-065X
Blake WiedenheftDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.ORCID 0000-0001-9297-5304

Funding

NCCAT: National Center for CryoEM Access and Training- Supplement for Windows 10 and FFIU24GM129539 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2018 to 2023
$53.9M
NCCAT: National Center for CryoEM Access and TrainingR24GM154192 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI EDWARD T ENG, Jeffrey S Kieft · 2024 to 2026
$21.0M
Surveillance genome sequencing to detect SARS-CoV-2 virus variants in MontanaP30GM140963 · NIGMS · UNIVERSITY OF MONTANA · PI SPRANG, STEPHEN R · 2021 to 2025
$6.9M
Structural and functional understanding of bacterial defense and viral counter defenseR35GM134867 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Blake A Wiedenheft · 2020 to 2026
$3.8M
Mechanisms of CRISPR-mediated immunity and applications beyond editingR00GM147842 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Andrew Santiago-Frangos · 2024 to 2026
$747k
Mechanisms of CRISPR-mediated immunity and applications beyond editingK99GM147842 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI SANTIAGO-FRANGOS, ANDREW · 2022 to 2023
$171k
NIGMS NIH HHS K99 GM147842NIGMS NIH HHS P30 GM140963NIGMS NIH HHS R00 GM147842NIGMS NIH HHS R24 GM154192NIGMS NIH HHS R35 GM134867NIGMS NIH HHS U24 GM129539
6 · The paper itself

Abstract

Cas1 and Cas2 are the hallmark proteins of prokaryotic adaptive immunity. However, these two proteins are often fused to other proteins and the functional association of these fusions often remain poorly understood. Here we purify Cas1 and the Cas2/3 fusion protein from

Indexed as

adaptationCasCRISPRimmunityintegrationPAM

Identifiers

PMID40661357
PMCPMC12259127

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.