Evidence map›Paper›PMID 40661351›Full record

ArticlebioRxiv : the preprint server for biology2025

A Multimodal Adaptive Optical Microscope For

Tian-Ming Fu, Gaoxiang Liu, Daniel E Milkie, Xiongtao Ruan, Frederik Görlitz, Yu Shi, Valentina Ferro, Nikita S Divekar, Wei Wang, Harrison M York and 40 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

50 authors.

Tian-Ming FuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.ORCID 0000-0001-6265-0859
Gaoxiang LiuDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Daniel E MilkieJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.ORCID 0000-0002-3917-6965
Xiongtao RuanDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0001-5322-3249
Frederik GörlitzDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Yu ShiLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Valentina FerroDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Nikita S DivekarDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0009-0006-9384-8407
Wei WangDepartment of Chemistry, University of Illinois Chicago; Chicago, IL 60607, USA.
Harrison M YorkMonash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton/Melbourne, VIC, 3800, Australia.ORCID 0000-0002-4534-8188
Velat KilicJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.ORCID 0000-0001-5594-1324
Matthew MuellerJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
Yajie LiangDepartment of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Timothy A DaugirdDepartment of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.ORCID 0000-0002-6313-0246
Maria J Gacha-GarayDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794-5215, USA.ORCID 0000-0002-9312-5237
Kathryn A LarkinDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794-5215, USA.
Rebecca C AdikesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794-5215, USA.ORCID 0000-0002-7526-8701
Nathaniel HarrisonDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Cyna ShirazinejadDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Samara WilliamsDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Jamison L NourseDepartment of Physiology and Biophysics, Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine 92697, USA.
Shu-Hsien SheuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.ORCID 0000-0003-0758-4654
Liang GaoJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
Tongchao LiDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Chandrani MondalDepartment of Medicine, Division of Hematology and Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Kemal AchourDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Wilmene HerculeDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Daniel StableyNeuroimaging Laboratory, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN, 38105, USA.ORCID 0000-0001-9187-6099
Kevin EmmerichWilmer Eye Institute and the Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Peng DongJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
David DrubinDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0003-3002-6271
Zhe J LiuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.ORCID 0000-0002-3592-3150
David ClaphamJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.ORCID 0000-0002-4459-9428
Jeff S MummWilmer Eye Institute and the Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.ORCID 0000-0002-2575-287X
Minoru KoyamaUniversity of Toronto, Department of Cell and Systems Biology, Scarborough, Ontario, Canada.ORCID 0000-0002-9774-9223
Alison KillileaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0002-8308-8196
Jose Javier Bravo-CorderoDepartment of Medicine, Division of Hematology and Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.ORCID 0000-0001-9481-1231
C Dirk KeeneUniversity of Washington BioRepository and Integrated Neuropathology (BRaIN) lab, Harborview Medical Center, Seattle, WA, USA.
Liqun LuoDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
Tom KirchhausenProgram in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115.ORCID 0000-0003-0559-893X
Medha M PathakDepartment of Physiology and Biophysics, Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine 92697, USA.
Senthil ArumugamMonash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton/Melbourne, VIC, 3800, Australia.ORCID 0000-0001-6733-4679
James K NunezDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0002-7396-6119
Ruixuan GaoDepartment of Chemistry, University of Illinois Chicago; Chicago, IL 60607, USA.ORCID 0000-0001-7137-2512
David Q MatusDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794-5215, USA.ORCID 0000-0002-1570-5025
Benjamin L MartinDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794-5215, USA.ORCID 0000-0001-5474-4492
Ian A SwinburneDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0003-4162-0508
Eric BetzigJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.ORCID 0000-0002-4192-1274
Wesley R LegantLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.ORCID 0000-0003-1827-5371
Srigokul UpadhyayulaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0001-6727-5883

Funding

THE TISCH CANCER INSTITUTE - CANCER CENTER SUPPORT GRANTP30CA196521 · NCI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Deanna L Benson · 2015 to 2026
$35.4M
University of Washington Alzheimer's Disease Research CenterP30AG066509 · NIA · UNIVERSITY OF WASHINGTON · PI SUMAN JAYADEV · 2020 to 2026
$29.0M
Nikon confocal system for improved spatio-dynamic analysis of endocytic trafficking eventsR35GM118149 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI DAVID G DRUBIN · 2016 to 2026
$12.0M
TRAINING PROGRAM IN CANCER THERAPYT32CA078207 · NCI · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI James J Manfredi · 1999 to 2026
$11.6M
Assembly of the Central Olfactory Networks in DrosophilaR01DC005982 · NIDCD · STANFORD UNIVERSITY · PI LIQUN LUO · 2003 to 2026
$8.1M
Visual Science Training ProgramT32EY007143 · NEI · JOHNS HOPKINS UNIVERSITY · PI Laura Ensign, JEREMY NATHANS · 1995 to 2026
$6.2M
VISUALIZATION OF SUBCELLULAR DYNAMICS IN MULTICELLULAR ORGANISMSR35GM130386 · NIGMS · BOSTON CHILDREN'S HOSPITAL · PI TOMAS KIRCHHAUSEN · 2019 to 2026
$4.0M
Building the brain: How mechanical forces shape human neural developmentDP2AT010376 · NCCIH · UNIVERSITY OF CALIFORNIA-IRVINE · PI PATHAK, MEDHA M · 2018 to 2020
$2.9M
Connecting the dots between single molecule dynamics and cell differentiationDP2GM136653 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LEGANT, WESLEY R. · 2019 to 2019
$2.3M
Piezo1 in neural stem cell mechano-regulationR01NS109810 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PATHAK, MEDHA M · 2018 to 2022
$2.2M
Novel models of Alzheimer's Disease and Related Disorders enabling systematic investigation of the therapeutic potential of transplanted hiPSC-derived cells and endogenous adult neural stem cellsR01OD020376 · OD · JOHNS HOPKINS UNIVERSITY · PI MUMM, JEFFREY · 2016 to 2020
$2.1M
Elucidating the mechanisms underlying cell cycle regulation of invasive behaviorR01GM121597 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI MATUS, DAVID · 2017 to 2021
$2.1M
NCCIH NIH HHS DP2 AT010376NCI NIH HHS P30 CA196521NCI NIH HHS R01 CA244780NCI NIH HHS T32 CA078207NEI NIH HHS T32 EY007143NIA NIH HHS P30 AG066509NIDCD NIH HHS R01 DC005982NIDCD NIH HHS R01 DC021710NIGMS NIH HHS DP2 GM136653NIGMS NIH HHS F32 GM133131NIGMS NIH HHS R01 GM121597NIGMS NIH HHS R35 GM118149NIGMS NIH HHS R35 GM130386NIGMS NIH HHS R35 GM150290NIGMS NIH HHS R35 GM155044NIH HHS R01 OD020376NINDS NIH HHS R01 NS109810Wellcome Trust
6 · The paper itself

Abstract

Understanding biological systems requires observing features and processes across vast spatial and temporal scales, spanning nanometers to centimeters and milliseconds to days, often using multiple imaging modalities within complex native microenvironments. Yet, achieving this comprehensive view is challenging because microscopes optimized for specific tasks typically lack versatility due to inherent optical and sample handling trade-offs, and frequently suffer performance degradation from sample-induced optical aberrations in multicellular contexts. Here, we present MOSAIC, a reconfigurable microscope that integrates multiple advanced imaging techniques including light-sheet, label-free, super-resolution, and multi-photon, all equipped with adaptive optics. MOSAIC enables non-invasive imaging of subcellular dynamics in both cultured cells and live multicellular organisms, nanoscale mapping of molecular architectures across millimeter-scale expanded tissues, and structural/functional neural imaging within live mice. MOSAIC facilitates correlative studies across biological scales within the same specimen, providing an integrated platform for broad biological investigation.

Identifiers

PMID40661351
PMCPMC12259214

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.