Evidence map›Paper›PMID 40660299›Full record

ArticleVirology journal2025

Serotype-specific host proteome remodeling in human foreskin fibroblasts during lytic HSV-1 and HSV-2 infection.

Xiaohong Pan, Jiaxin Xie, Zhidan Zhang, Xiaomei Guo, Jixiong Li, Di Lin, Ying Qian, Jingwen Xu, Yunzhang Hu, Jiandong Shi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaohong Pan *Yunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Jiaxin Xie *Yunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Zhidan ZhangYunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Xiaomei GuoYunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Jixiong LiYunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Di LinYunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Ying QianYunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Jingwen XuYunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Yunzhang HuYunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China. huyunzhangym@126.com.
Jiandong ShiYunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China. shijiandong@imbcams.com.cn.ORCID 0000-0002-3329-1014

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) exhibit distinct clinical manifestations, yet the molecular basis of their serotype-specific pathogenicity remains unclear. This study presents a comparative proteomic analysis of human foreskin fibroblast (HFF-1) cells during lytic HSV-1 and HSV-2 infections to elucidate host-pathogen interactions driving differential virulence. Using data-independent acquisition mass spectrometry (DIA-MS), we identified 280 and 219 differentially expressed proteins (DEPs) in HSV-1- and HSV-2-infected cells, respectively. Key DEPs revealed serotype-specific modulation: HSV-1 upregulated antiviral effectors (ISG20, IRF7) while downregulating chemokine signaling (CXCL12, DEF8) and promoting lipid metabolism (PTDSS1). In contrast, HSV-2 upregulated inflammatory effectors (IGHV3-9, SERPINA1), enhanced NF-κB signaling (BCL3), and altered glycometabolism (GYS1, FBN1). Pathway enrichment analysis showed that HSV-1 suppressed inflammatory and antigen presentation pathways to evade immune responses, whereas HSV-2 induced stronger pro-inflammatory responses and metabolic reprogramming related to lipid and glycometabolism. Overall, these findings provide a proteomic roadmap for understanding serotype-specific pathogenesis.

Indexed as

FibroblastsForeskinHerpes SimplexHerpesvirus 1, HumanHerpesvirus 2, HumanHost-Pathogen InteractionsProteomeCell LineHumansMaleProteomicsSerogroupProteomeAntiviral immunityHSV-1HSV-2Metabolic reprogrammingProteomicsViral pathogenesis

Identifiers

PMID40660299
PMCPMC12257817

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.