Evidence map›Paper›PMID 40660090›Full record

ArticleJournal of extracellular vesicles2025

Improved Accessibility of Extracellular Vesicle Surface Molecules Upon Partial Removal of the Protein Corona by High Ionic Strength.

András I Försönits, Eszter Á Tóth, Sára Jezsoviczky, Tünde Bárkai, Delaram Khamari, Alicia Galinsoga, Panna Királyhidi, Ágnes Kittel, Júlia Fazakas, Dorina Lenzinger and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Protective Effects and Mechanisms of Extracellular Vesicles in Remote Ischemic Preconditioning on Renal Injury Induced by Inflammation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

András I FörsönitsDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Eszter Á TóthDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Sára JezsoviczkyDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Tünde BárkaiDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Delaram KhamariDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Alicia GalinsogaDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Panna KirályhidiDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Ágnes KittelDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Júlia FazakasDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Dorina LenzingerDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Hargita HegyesiDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Xabier OsteikoetxeaDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.ORCID https://orcid.org/0000-0003-3628-0174
Tamás VisnovitzDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Krisztina PálócziDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Szilvia BőszeDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Edit I BuzásDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.ORCID https://orcid.org/0000-0002-3744-206X

Funding

Edit BuzasHungarian National Research, Development and Innovation Office (NKFIH)János Bolyai Scholarship (Tamás Visnovitz)Magyar Tudományos AkadémiaSemmelweis Egyetem STIA 2020 KFI
6 · The paper itself

Abstract

Recent studies have confirmed that a biomolecular corona forms around extracellular vesicles (EVs) in biofluids. However, there is limited data on how this adsorbed corona affects the accessibility of EV surface molecules. Here, we investigated various potential corona-stripping conditions for their ability to affect the immune detection of EVs. First, we artificially formed an EV corona around nascent HEK293T-PalmGFP cell-derived large EVs (lEVs) by incubating them with Cy5-labelled human plasma proteins. The co-localisation rate of plasma proteins and lEVs decreased significantly upon high-salt washing with NaCl, LiCl and KCl solutions, suggesting a considerable removal of the corona components. Additional evidence for corona modification was a significantly increased fluorescent annexin V binding to plasma lEVs and annexin V affinity capture of both THP1- and blood plasma-derived lEVs upon high-salt washing. A similar effect of high ionic strength was observed when THP1 lEVs were separated from a serum-containing medium, which allowed for corona formation, but not when EVs were produced under serum-free conditions. Using a MACSPlex kit and high-salt washing for small EVs from plasma and THP1 conditioned medium, we also demonstrated significantly improved immunodetection of 15 and 9 out of 37 surface markers, respectively. In this Technical Note, we present evidence that modifying the protein corona around EVs can significantly affect the immune detection of specific EV markers.

Indexed as

Extracellular VesiclesProtein CoronaBlood ProteinsHEK293 CellsHumansOsmolar ConcentrationBlood ProteinsProtein Coronablood plasmaextracellular vesicleflow cytometryimmunolabellingprotein corona

Identifiers

PMID40660090
PMCPMC12259388

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.