Evidence map›Paper›PMID 40659793›Full record

ArticleScientific reports2025

Molecular characterization of multidrug-resistant and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolated from Sonali chicken meat in Bangladesh.

Muhammad Ali, Jahid Hasan Tipu, Obaidul Islam, Ahsan Raquib, Ferdaus Mohd Altaf Hossain, Mohammad Al Mamun, Md Masudur Rahman, Abu Hena Mustafa Kamal, Monira Noor

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

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4citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Muhammad Ali *College of Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Jahid Hasan Tipu *Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.
Obaidul Islam *Laboratory of Veterinary Epidemiology, College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Ahsan RaquibDepartment of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PEI, C1A 4P3, Canada.
Ferdaus Mohd Altaf HossainDepartment of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.
Mohammad Al MamunDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.
Md Masudur RahmanDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.
Abu Hena Mustafa KamalDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.
Monira NoorDepartment of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh. noorm.dpp@sau.ac.bd.

Funding

World's Poultry Science Association-Bangladesh Branch (WPSA-BB) Open Research Grants 2023
6 · The paper itself

Abstract

Antimicrobial resistance (AMR) is a growing global public health concern, with contamination of poultry meat by multidrug-resistant and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) posing serious risks to food safety and public health. Therefore, this study aimed to isolate and characterize the multidrug-resistant E. coli harboring β-lactam resistance genes from raw Sonali chicken meat samples collected from retail poultry shops across six upazilas of the Narsingdi district in Bangladesh. A total of 390 meat swab samples were collected and analyzed between July and December 2023. E. coli was detected in 68.21% of the isolates, with 7.89% classified as enteropathogenic E. coli (EPEC) and 92.11% as non-EPEC. Antimicrobial susceptibility testing by disc diffusion method revealed complete resistance to ampicillin (100%), with high resistance to erythromycin (87.59%) and third-generation cephalosporins. Furthermore, the lowest resistance was observed for amoxicillin-clavulanate (3.01%). ESBL production was identified in 41.73% of the isolates. On the other hand, non-EPEC strains showed a significantly higher prevalence of ESBL production (p < 0.05). Genotypic analysis of β-lactamase genes in ESBL-positive isolates demonstrated that blaTEM (63.06%) and blaCTX-M-2a (60.36%) were the most prevalent. Additionally, co-occurrence of multiple β-lactamase genes was observed in 54.95% of isolates, with blaTEM, blaCTX-M-2a, and blaOXA-1 being the most common combination. Furthermore, resistance genes for AmpC (blaCMY, 27.07%) and metallo-β-lactamase (blaNDM-1, 9.02%) were exclusively detected in non-EPEC isolates. Significant positive correlations were observed among β-lactamase genes, highlighting the complexity of resistance mechanisms. Our results demonstrate the necessity of judiciously using antibiotics in Sonali chickens to prevent the emergence of resistant bacterial strains.

Indexed as

beta-LactamasesDrug Resistance, Multiple, BacterialEscherichia coliMeatAnimalsAnti-Bacterial AgentsBangladeshChickensFood MicrobiologyMicrobial Sensitivity TestsAnti-Bacterial Agentsbeta-LactamasesBangladeshEscherichia coli (E. coli)Extended-spectrum beta-lactamase (ESBL)Food safetyMultidrug-resistantSonali chicken

Identifiers

PMID40659793
PMCPMC12260069

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.