Evidence map›Paper›PMID 40659783›Full record

ArticleScientific reports2025

CENPF overexpression in bladder cancer cells enhances proliferation, migration, invasion, and apoptosis.

Ruixiang Song, Xin Chen, Zhensheng Zhang, Huiqing Wang, Jinshan Xu, Shuxiong Zeng, Wentao Zhang, Zhaoyang Sheng, Xudong Yao

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Ruixiang Song *Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
Xin Chen *Department of Urology, Zhejiang Qiushi Cardiovascular Hospital, Hangzhou, China.
Zhensheng Zhang *Department of Urology, Changhai Hospital, Naval Medical University, Shanghai, China.
Huiqing WangDepartment of Urology, Changhai Hospital, Naval Medical University, Shanghai, China.
Jinshan XuDepartment of Urology, Changhai Hospital, Naval Medical University, Shanghai, China.
Shuxiong ZengDepartment of Urology, Changhai Hospital, Naval Medical University, Shanghai, China.
Wentao ZhangDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
Zhaoyang ShengDepartment of Urology, Changhai Hospital, Naval Medical University, Shanghai, China. 1113165278@qq.com.
Xudong YaoDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China. yaoxudong1967@163.com.

Funding

Experimental Animal Fund of Shanghai Science and Technology Commission No. 22140903800National Key Research and Development Program of China 2022YFB3804504National Natural Science Foundation of China, Youth Project Nos. 82101838, 82203367, 82203505, and 82203403
6 · The paper itself

Abstract

Bladder cancer has been considered as one of the most common urinary malignancies. Growing evidence has indicated that Centromere Protein F (CENPF) is a promising molecular biomarker for many human malignant tumors. However, the role of CENPF in bladder cancer (BC) proliferation, migration, invasion, cell cycle and apoptosis thereof remain unclear. In the present study, high-throughput sequencing technology and bioinformatics analysis were conducted to identify mRNAs profiles in 10 pairs of bladder cancer tissues and adjacent noncancerous tissues. CENPF was overexpression in bladder cancer tissues, and higher in MIBC than NMIBC. We investigated the expression of CENPF in 30 other pairs bladder cancers tissues, and the results were in accordance with the sequencing results. Furthermore, Immunohistochemical staining, showed that strong intensity of CENPF in BC tissues than normal tissues. Increased staining of CENPF was detected of tumor cells in MIBC compared with NMIBC. This suggests that CENPF might be highly expressed in aggressive and invasive tumor cells. Subsequently, in vitro functional experiments also demonstrated that the siRNA interference of CENPF expression significantly weakened the proliferation, migration, invasion and apoptosis of BC cells, and the cells were arrested in the G2/S phase in Cell cycle. Moreover, functional enrichment analyses, lncRNA-miRNA-mRNA, and protein-protein interaction networks revealed that CENPF was potentially involved in carcinogenesis and evolution of bladder cancer. Taken together, these results demonstrated that CENPF may serve as a potential biomarker of tumor occurrence, progression, and even prognosis for bladder cancer. However, further research is needed to be further clarified the pathway mechanisms of CENPF in bladder cancer.

Indexed as

ApoptosisCell MovementChromosomal Proteins, Non-HistoneMicrofilament ProteinsUrinary Bladder NeoplasmsBiomarkers, TumorCell Line, TumorCell ProliferationFemaleGene Expression Regulation, NeoplasticHumansMaleMicroRNAsMiddle AgedNeoplasm InvasivenessRNA, Long NoncodingBiomarkers, Tumorcentromere protein FChromosomal Proteins, Non-HistoneMicrofilament ProteinsMicroRNAsRNA, Long NoncodingBiomarkerBladder cancerCell functional experimentsCENPF

Identifiers

PMID40659783
PMCPMC12259981

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