Evidence map›Paper›PMID 40659700›Full record

ArticleScientific reports2025

Beneficial effects of carvacrol loaded phytosomes on enhancing cryotolerance of Buffalo semen following cryopreservation.

Wael A Khalil, Salwa A Elkhamy, Mohamed M Hegazy, Mahmoud A E Hassan, Ahmed Mowafy Tafish, Sameh A Abdelnour, Mostafa A El-Harairy

Abstract read
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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wael A Khalil *Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt. w-khalil@mans.edu.eg.
Salwa A Elkhamy *Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt.
Mohamed M HegazyAnimal Production Research Institute, Agriculture Research Centre, Ministry of Agriculture, Dokki, Giza, 12619, Egypt.
Mahmoud A E HassanAnimal Production Research Institute, Agriculture Research Centre, Ministry of Agriculture, Dokki, Giza, 12619, Egypt.
Ahmed Mowafy TafishDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Horus University, New Damietta, 34518, Egypt.
Sameh A AbdelnourDepartment of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt. samehtimor86@gmail.com.
Mostafa A El-HarairyDepartment of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sperm cryopreservation technology underpins genetic advancement in animal breeding and ongoing research aims to minimize cryoinjuries. This study aimed to develop carvacrol-loaded phytosomes (CLNPs) to enhance the physicochemical properties of carvacrol in aqueous cryopreservation extenders. Semen samples from five buffalo bulls were collected, extended and cryopreserved with varying CLNPs concentrations (0, 2.5, 5, 10, and 20 µg/mL). The freshly prepared CLNPs exhibited an average particle size of 286.7 ± 11.27 nm, a polydispersity index of 0.189 ± 0.05, and a zeta potential of - 11.4 ± 0.26 mV. Supplementing the freezing media with CLNPs significantly enhanced sperm progressive motility, viability, and plasma membrane integrity after both equilibration (5 °C for 4 h) and thawing. Furthermore, sperm kinematic parameters were significantly higher in all CLNPs-treated groups (P < 0.05). Compared to the CLNPs-free extender, CLNPs supplementation significantly reduced the percentage of dead sperm with intact acrosomes and increased the percentage of live sperm with intact acrosomes (P < 0.001). Post-thaw oxidative stress markers, including H

Indexed as

CryopreservationCryoprotective AgentsCymenesSemenSemen PreservationAnimalsBuffaloesMaleOxidative StressPhytosomesSpermatozoaSperm MotilitycarvacrolCryoprotective AgentsCymenesPhytosomesBuffalo semenCarvacrol-loaded phytosomesMicrobiotaMitochondrial functionMolecular dockingRedox Status

Identifiers

PMID40659700
PMCPMC12259984

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.