Comparative Analysis of Signature Sequences from Adenylation Domains Situated within Bacterial-Origin Nonribosomal Peptide Synthetase Modules.

Weina Gao, Zhishen Zhang, Huiying Yu, Xin Li, Chunshan Quan, Yun Xue, Pengchao Zhao

Abstract readComparative Study

In one paragraph

Article in Journal of microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Weina GaoCollege of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, P.R. China.

Zhishen ZhangCollege of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, P.R. China.

Huiying YuShanxi Key Laboratory of Yuncheng Salt Lake Ecological Protection and Resource Utilization, Yuncheng University, Yuncheng 044000, P.R. China.

Xin LiShanxi Key Laboratory of Yuncheng Salt Lake Ecological Protection and Resource Utilization, Yuncheng University, Yuncheng 044000, P.R. China.

Chunshan QuanDepartment of Life Science, Dalian Nationalities University, Dalian 116600, P.R. China.

Yun XueCollege of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, P.R. China.

Pengchao ZhaoCollege of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nonribosomal peptides are assembled by large enzymes that contain multiple active sites, which function in a modular manner. The adenylation (A) domains present within typical nonribosomal peptide synthetase (NRPS) modules contain specificity-conferring codes or signature sequences (SNSs). In this study, we obtained 2051 A domain sequences from 67 bacterial species. Their alignment and clustering identified 508 SNSs. Over 80% of the SNSs displayed distinct specificity for 36 proteinogenic and nonproteinogenic α-amino acid moieties (α-AAMs). Furthermore, modifications such as

Indexed as

BacteriaPeptide SynthasesAmino AcidsAmino Acid SequenceBacterial ProteinsCatalytic DomainProtein DomainsSequence AlignmentSubstrate SpecificityAmino AcidsBacterial Proteinsnon-ribosomal peptide synthasePeptide Synthasesadenylation domaindistribution algorithmsequence alignmentsignature sequencesα-Amino acid moiety

Identifiers

PMID40659553

DOI10.4014/jmb.2503.02030

PMCPMC12283262

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