Evidence map›Paper›PMID 40659489›Full record

ArticleLife science alliance2025

A lyophilized open-source RT-LAMP assay for molecular diagnostics in resource-limited settings.

Martin Matl, Max J Kellner, Felix Ansah, Irina Grishkovskaya, Dominik Handler, Robert Heinen, Benedikt Bauer, Luis Menéndez-Arias, Thomas O Auer, Lucia L Prieto-Godino and 5 more

Abstract read
In one paragraph

Article in Life science alliance, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Martin MatlResearch Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.ORCID 0000-0002-7215-3386
Max J KellnerResearch Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Felix AnsahWest African Center for Cell Biology of Infectious Pathogens (WACCBIP), College of Basic and Applied Sciences, University of Ghana, Legon, Ghana.
Irina GrishkovskayaResearch Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Dominik HandlerInstitute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Vienna, Austria.ORCID 0000-0002-1059-4960
Robert HeinenResearch Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Benedikt BauerResearch Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
Luis Menéndez-AriasCentro de Biología Molecular 'Severo Ochoa' (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain.
Thomas O AuerDepartment of Biology, University of Fribourg, Fribourg, Switzerland.ORCID 0000-0003-3442-0567
Lucia L Prieto-GodinoTReND in Africa, Brighton, UK.
Josef M PenningerInstitute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Vienna, Austria.
Vienna Covid-19 Detection Initiative (VCDI)
Gordon A AwandareWest African Center for Cell Biology of Infectious Pathogens (WACCBIP), College of Basic and Applied Sciences, University of Ghana, Legon, Ghana gawandare@ug.edu.gh.ORCID 0000-0002-8793-3641
Julius BrenneckeInstitute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Vienna, Austria julius.brennecke@imba.oeaw.ac.at.ORCID 0000-0002-5141-0814
Andrea PauliResearch Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria andrea.pauli@imp.ac.at.ORCID 0000-0001-9646-2303

Funding

Wellcome Trust
6 · The paper itself

Abstract

A critical bottleneck for equitable access to population-scale molecular diagnostics is the limited availability of rapid, inexpensive point-of-care tests, especially in low- and middle-income countries. Here, we developed an open-source reverse transcription loop-mediated isothermal amplification (RT-LAMP) molecular assay for pathogen detection. It is based on nonproprietary enzymes, namely, HIV-1 reverse transcriptase,

Indexed as

COVID-19Molecular Diagnostic TechniquesNucleic Acid Amplification TechniquesSARS-CoV-2Freeze DryingHIV-1HumansPoint-of-Care TestingResource-Limited SettingsRNA, ViralSensitivity and SpecificityRNA, Viral

Identifiers

PMID40659489
PMCPMC12260655

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.