ArticleJournal of the American Chemical Society2025
Photoswitchable Fluorescence of Peptide-Based Hemipiperazines Inside of Living Cells.
Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Activated excited-state isomerisation in a hemipiperazine photoswitch.Chemical communications (Cambridge, England) · 2026Article
- Dynamic Control of Nucleic Acids Self-Assembly and Expression Using Photoswitches.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Thermally Bistable Stiff Stilbene Photoswitches and Polymer Applications.Chemical record (New York, N.Y.) · 2026Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photoswitchable fluorophores that can be toggled with visible light are extremely useful for applications in super-resolution imaging. However, most small-molecule photoswitches suffer from poor aqueous solubility and limited biocompatibility and require UV-light activation. Here, we report a novel class of biocompatible, visible-light-responsive fluorophores based on hemipiperazine (HPI) scaffolds with annulated π-systems─indolo-hemipiperazines (IndHPIs) and pyrrolo-hemipiperazines (PyrHPIs). These compounds display large Stokes shifts (up to 135 nm), reversible photoisomerization with up to 620 nm wavelength of light, and, in particular examples, enhanced fluorescence quantum yields and switching thereof, augmented by internal H-bonding. Selected compounds demonstrated excellent thermal stability of their E-isomers, with half-lives of up to ∼13,000 h at 50 °C, and high fatigue resistance under repeated switching cycles. Notably, certain IndHPIs are efficiently internalized by living cells and exhibit a reversible modulation of fluorescence upon irradiation. Further mechanistic studies revealed that
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Registered trials
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