ArticleMethods in molecular biology (Clifton, N.J.)2026
Generation of the Nonintegrated Human Bilaminar Embryo Model (Bilaminoid) from Naïve Pluripotent Stem Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Human embryogenesis has been problematic to study due to technical and ethical issues. Recently, a human embryo model generated from pluripotent stem cells (PSCs) to mimic human embryogenesis, which has attracted attention as an invaluable tool for studying human embryonic development.We have successfully developed a method to efficiently induce the differentiation of naïve human PSCs, which correspond to preimplantation epiblasts, into extraembryonic cells of the blastocyst. Furthermore, by combining these cells, we developed a novel nonintegrated human embryo model called "bilaminoid" that reproduces development from preimplantation to peri-gastrulation stages. Here, we describe a detailed protocol for the bilaminoid formation.
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