Evidence map›Paper›PMID 40657757›Full record

ArticleAnnals of surgery2025

Mosaic Loss of Y Chromosome Defines a Proximal Tubular Cell State Associated with Recovery from DGF and of Allograft Quality and Functional Reserve Post DCD Kidney Transplantation.

Andrew F Malone, Rohan Paul, Haikuo Li, Aidan Leckie-Harre, Haojia Wu, Nicolas Ledru, Gwen McPherson, Hollie B Davies, Tracy Doty, Alan C Farney and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of surgery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Andrew F MaloneDepartment of Nephrology, St Vincent's University Hospital, University College Dublin, Dublin, Ireland.
Rohan PaulDivision of Nephrology, Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, Missouri.
Haikuo LiDivision of Nephrology, Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, Missouri.
Aidan Leckie-HarreDivision of Nephrology, Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, Missouri.
Haojia WuDivision of Nephrology, Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, Missouri.
Nicolas LedruDivision of Nephrology, Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, Missouri.
Gwen McPhersonDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Hollie B DaviesDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Tracy DotyDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Alan C FarneyDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Collee C JayDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Emily McCrackenDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Christopher J WebbDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Alejandra Mena-GutierrezAtrium Health Wake Forest Baptist Health, Abdominal Organ Transplant Program, Winston Salem, USA.
Mohammad Kazem FallahzadehAtrium Health Wake Forest Baptist Health, Abdominal Organ Transplant Program, Winston Salem, USA.
Amber Reeves-DanielAtrium Health Wake Forest Baptist Health, Abdominal Organ Transplant Program, Winston Salem, USA.
Natalia V SakhovskajaAtrium Health Wake Forest Baptist Health, Abdominal Organ Transplant Program, Winston Salem, USA.
Steve J WalkerWake Forest School of Medicine, Winston Salem, USA.
Alexandra R MonettiWake Forest School of Medicine, Winston Salem, USA.
John R SavinoWake Forest School of Medicine, Winston Salem, USA.
Alexei V MikhailovWake Forest School of Medicine, Winston Salem, USA.
Robert J StrattaDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Lori N ByersDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Amish AsthanaDepartment of Surgery, Section of Transplantation, Winston Salem, USA.
Benjamin D HumphreysDivision of Nephrology, Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, Missouri.
Giuseppe OrlandoDepartment of Surgery, Section of Transplantation, Winston Salem, USA.ORCID 0000-0002-6460-7974

Funding

Washington University Chronic KidneyDisease National Resource CenterU54DK137332 · NIDDK · WASHINGTON UNIVERSITY · PI BENJAMIN D. HUMPHREYS · 2023 to 2026
$4.5M
Understanding Myofibroblast Progenitor Fate and Function in Renal FibrosisR01DK103740 · NIDDK · WASHINGTON UNIVERSITY · PI BENJAMIN D. HUMPHREYS · 2015 to 2026
$3.9M
Proximal Tubule Alternative Splicing and Progression of Diabetic Kidney DiseaseR01DK136663 · NIDDK · WASHINGTON UNIVERSITY · PI BENJAMIN D. HUMPHREYS · 2025 to 2026
$1.1M
NIDDK NIH HHS R01 DK103740NIDDK NIH HHS R01 DK136663NIDDK NIH HHS U54 DK137332
6 · The paper itself

Abstract

objectiveTo determine the drivers of proximal tubular cell regeneration and repair over time in the setting of recovery from delayed graft function (DGF) post donation after cardiac death (DCD) kidney transplantation.

backgroundDCD Kidney allografts are at increased risk of graft loss. Despite this, due to organ shortages, DCD transplantation is increasing, which offers a novel and valuable platform for the study of adaptive/maladaptive repair mechanisms after injury.

methodsWe analyzed the dynamic transcriptional changes in serial biopsies of transplanted DCD kidneys to better understand proximal tubular cell repair and regeneration in DGF at the cellular level. We also quantified loss of Y chromosome in these kidneys to determine the impact of this phenomenon on DGF.

resultsOne cell state associated with recovery from DGF had a high loss of Y chromosome fraction (29%) compared to pre-transplant healthy proximal tubules (17%) and differentially expressed APOE. A proximal tubule cell state associated with progression to DGF was defined by injury markers VCAM1 and HAVCR1 and proinflammatory and proliferative genes CCL2 and MYC and accounted for over 50% of proximal tubules in time 0 DGF kidney biopsies.

conclusionsTaken together, our dataset of serial biopsies from DCD kidney transplants reveals insights into mechanisms driving injury and repair in the setting of DGF and offers a signature of allograft quality and functional reserve that may optimize allocation at the time of procurement.

Indexed as

DCDDGFkidney transplantationproximal tubular cellsSignature of organ qualitysingle cell analysisY chromosome

Identifiers

PMID40657757
PMCPMC13310177

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