Evidence map›Paper›PMID 40657538›Full record

ArticleBio-protocol2025

Method for Extracellular Electrochemical Impedance Spectroscopy on Epithelial Cell Monolayers.

Athena J Chien, Colby F Lewallen, Hanna Khor, Analia Vazquez Cegla, Rongming Guo, Adrienne L Watson, Chris Hatcher, Nael A McCarty, Kapil Bharti, Craig R Forest

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Athena J ChienWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Colby F LewallenOcular and Stem Cell Translational Research Section, Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
Hanna KhorSchool of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Analia Vazquez CeglaDivision of Pulmonology, Asthma, Cystic Fibrosis, and Sleep, Department of Pediatrics, Emory + Children's Center for Cystic Fibrosis and Airways Disease Research, Emory University, Atlanta, GA, USA.
Rongming GuoG.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Adrienne L WatsonWorld Precision Instruments, Sarasota, FL, USA.
Chris HatcherWorld Precision Instruments, Sarasota, FL, USA.
Nael A McCartyDivision of Pulmonology, Asthma, Cystic Fibrosis, and Sleep, Department of Pediatrics, Emory + Children's Center for Cystic Fibrosis and Airways Disease Research, Emory University, Atlanta, GA, USA.
Kapil BhartiOcular and Stem Cell Translational Research Section, Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
Craig R ForestWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Funding

Novel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7M
High-throughput robotic analysis of integrated neuronal phenotypesR01EY023173 · NEI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2012 to 2016
$4.3M
Automated cell-type-specific electrophysiology for understanding circuit dysregulation in Alzheimer's DiseaseRF1AG079269 · NIA · EMORY UNIVERSITY · PI FOREST, CRAIG, ROWAN, MATTHEW J.M. · 2022 to 2024
$2.4M
Scalable Cell- and Circuit-Targeted ElectrophysiologyR01NS102727 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2017 to 2020
$2.2M
In-vivo circuit activity measurement at single cell, sub-threshold resolutionU01MH106027 · NIMH · GEORGIA INSTITUTE OF TECHNOLOGY · PI FOREST, CRAIG, STANLEY, GARRETT B. · 2014 to 2016
$1.5M
NEI NIH HHS R01 EY023173NIA NIH HHS RF1 AG079269NIDA NIH HHS R01 DA029639NIMH NIH HHS U01 MH106027NINDS NIH HHS R01 NS102727
6 · The paper itself

Abstract

Epithelial tissues form barriers to the flow of ions, nutrients, waste products, bacteria, and viruses. The conventional electrophysiology measurement of transepithelial resistance (TEER/TER) can quantify epithelial barrier integrity, but does not capture all the electrical behavior of the tissue or provide insight into membrane-specific properties. Electrochemical impedance spectroscopy, in addition to measurement of TER, enables measurement of transepithelial capacitance (TEC) and a ratio of electrical time constants for the tissue, which we term the membrane ratio. This protocol describes how to perform galvanostatic electrochemical impedance spectroscopy on epithelia using commercially available cell culture inserts and chambers, detailing the apparatus, electrical signal, fitting technique, and error quantification. The measurement can be performed in under 1 min on commercially available cell culture inserts and electrophysiology chambers using instrumentation capable of galvanostatic sinusoidal signal processing (4 μA amplitude, 2 Hz to 50 kHz). All fits to the model have less than 10 Ω mean absolute error, revealing repeatable values distinct for each cell type. On representative retinal pigment (n = 3) and bronchiolar epithelial samples (n = 4), TER measurements were 500-667 Ω·cm

Indexed as

Barrier integrityElectrochemical impedance spectroscopyElectrophysiologyEpithelial cell monolayersTransepithelial capacitanceTransepithelial electrical resistance (TEER)Transport dynamics

Identifiers

PMID40657538
PMCPMC12254589

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.