Evidence map›Paper›PMID 40656557›Full record

ArticleBiology methods & protocols2025

Cost-effective production of

Kirsten H Benidickson, Kyle F Symonds, Wayne A Snedden, William C Plaxton

Abstract read
In one paragraph

Article in Biology methods & protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kirsten H BenidicksonDepartment of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6.ORCID https://orcid.org/0009-0000-0247-7521
Kyle F SymondsDepartment of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6.ORCID https://orcid.org/0009-0009-9765-5831
Wayne A SneddenDepartment of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6.ORCID https://orcid.org/0000-0001-9564-3057
William C PlaxtonDepartment of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6.ORCID https://orcid.org/0000-0002-8447-7249

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

γ-aminobutyrate (GABA) is a non-proteinogenic amino acid produced by glutamate decarboxylase (GAD) that functions as a vital neurotransmitter in animals, and as an important metabolite and signaling molecule in plants and microbes. "GABase" consists of a mixture of recombinant GABA transaminase (GABA-T) and succinic semialdehyde dehydrogenase (SSDH) that is widely used for spectrophotometric quantification of glutamate decarboxylase (GAD) activity or GABA levels in tissue extracts. Both can be conveniently monitored at 340 nm owing to the sequential conversion of GABA into succinate by GABA-T and SSDH, and concomitant reduction of NADP

Indexed as

GABA transaminaseglutamate decarboxylasemicroplate protocolrecombinant protein expression and purificationspectrophotometrysuccinic semialdehyde dehydrogenaseγ-aminobutyrate (GABA)

Identifiers

PMID40656557
PMCPMC12255878

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.