ArticleCytotechnology2025
Modulatory roles of bone morphogenetic protein 2 and asporin in osteo/cementoblast differentiation potential of human periodontal ligament stem cells: a multivariate model analysis.
Article in Cytotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the molecular mechanisms regulating osteo/cementogenic differentiation is critical for optimizing cell-based strategies in periodontal regeneration. This study employed multivariate statistical modeling to investigate, in vitro, the interaction between bone morphogenetic protein 2 (BMP2) and asporin (ASPN) in human periodontal ligament stem cells (hPDLSCs) and its impact on mineralization potential. Four primary hPDLSC populations-two with high osteo/cementogenic potential (HOP) and two with low potential (LOP)-were cultured under standard (SDM) and osteo/cementogenic (OM) conditions. Mineralization was assessed using Alizarin Red Staining (AR-S) and alkaline phosphatase (ALP) activity, while gene expression of ASPN, BMP2, osteocalcin (OCN), and runt-related transcription factor 2 (RUNX2) was quantified by quantitative real-time polymerase chain reaction (qRT-PCR). BMP2 protein levels were measured using the Luminex system. Data analysis incorporated Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), Pearson correlations, correlation networks, and multivariate regression models in RStudio. HOP cells exhibited higher mineralization and BMP2 expression compared to LOP cells (p < 0.05). ASPN negatively correlated with BMP2 expression and mineralization, particularly in LOP cells (p < 0.01), reinforcing its inhibitory role. Multivariate modeling identified BMP2 as a key positive regulator of osteo/cementogenesis, while ASPN emerged as a significant inhibitory factor (p < 0.001). This study highlights the potential of multivariate models as powerful tools for uncovering molecular interactions and identifying novel therapeutic targets, paving the way for advancements in periodontal regeneration and cell-based therapies. Graphical abstract: Created in BioRender. Sacramento, C. (2025). Supplementary Information: The online version contains supplementary material available at 10.1007/s10616-025-00810-x.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.